Toxicological studies of a feed additive based on live yeast saccharomyces cerevisiae on laboratory animals

УДК 619:001.891.53:582.282.23:636.028
DOI 10.33861/2071-8020-2023-5-18-21

Malkov S. V., Krasnoperov A. S., Oparina O. Yu., Belousov A. I.

Summary. When determining the acute and chronic toxicity of a feed additive based on live yeast Saccharomyces cerevisiae var. boulardii, symptom complex of poisoning in laboratory animals was not observed. The rats had no changes in the intake of feed and water, the color of visible mucous membranes, reactions to sound and pain stimuli. The values of body temperature, pulse rate and number of respiratory movements remained within the reference values. No animal deaths were detected during the experiment. A three-month period for determining chronic toxicity established insignificant changes in hematological parameters of experimental and control individuals. An increase in the absolute values of the hematocrit level, the number of erythrocytes and leukocytes was observed. This is a sign of the physiological «adjustment» of the body of laboratory rats with the inclusion of compensatory mechanisms for maintaining the balance of the body. These locomotions did not exceed physiological limits. Background values of biochemical parameters of blood serum of laboratory rats were within the reference values. The total protein content is 75,24±1,77 g/l, glucose - 4,84±0,18 mmol/l, creatinine - 67,96±4,99 mmol/l, urea -4,64±0,39 mmol/l, cholesterol - 2,13±0,21 mmol/l. An increase in the activity of serum enzymes was revealed: alkaline phosphatase - 191,13±34,27 U/l, aspartate aminotransferase (AST) - 170,25±15,78 U/l. This is not critical for the physiological norm of rodents. Long-term use of the feed additive in laboratory animals activated protein metabolism. The amount of total protein in the blood serum of individuals of the experimental groups increased by 2,8-4,5%. This indicates a better assimilation of feed nutrients.

Keywords: feed additive, live yeast, Saccharomyces cerevisiae var. boulardii, preclinical studies, acute toxicity, chronic toxicity, live weight, hematological studies, biochemical studies, laboratory rats.

References:

1. GOST R 51232-98. Voda pityevaya. Obshchie trebovaniya k organizatsii i metodam kontrolya kachestva [Drinking water. General requirements for organization and methods of quality control].

2. GOST 34566-2019. Kombikorma polnoratsionnye dlya laboratornykh zhivotnykh. Tekhnicheskie usloviya [Complete feed for laboratory animals. Specifications].

3 Krasnoperov A. S. et al. Izuchenie khronicheskoy toksichnosti kormovoy dobavki B-set na laboratornykh zhivotnykh [Study of the chronic toxicity of the feed additive B-set in laboratory animals]. - 2017: 235-240.

4. Lomako V. V. Vliyanie raznykh rezhimov okhlazhdeniya (kraniotserebralnoy i immersionnoy gipotermii, poverkhnostnykh ritmicheskikh i ekstremalnykh kholodovykh vozdeystviy) na leykotsitarnye pokazateli krovi krys [Influence of different cooling modes (craniocerebral and immersion hypothermia, superficial rhythmic and extreme cold effects) on leukocyte blood parameters in rats]. -Problemy kriobiologii i kriomeditsiny. - 2018 (28 (4). - pp. 293-310.

5. Metodicheskie rekomendatsii po soderzhaniyu laboratornykh zhivotnykh v vivariyakh nauchno-issledovatel’skikh institutov i uchebnykh zavedeniy [Guidelines for keeping laboratory animals in vivariums of research institutes and educational institutions]. - 2009: 29 p.

6. GOST 32644-2014. Metody ispytaniya po vozdeystviyu khimicheskoy produktsii na organizm cheloveka. Ostraya peroralnaya toksichnost - metod opredeleniya klassa ostroy toksichnosti [Test methods for the effects of chemical products on the human organism. Acute oral toxicity - method for determining acute toxicity class].

7. Mironov A. N. Rukovodstvo po provedeniyu doklinicheskikh issledovaniy lekarstvennykh sredstv: chast pervaya [Guidelines for conducting preclinical drug trials: part one]. - Grif i K. - 2012. - 944 p.

8 Shkuratova I. A. et al. Opredelenie ostroy i khronicheskoy toksichnosti preparata «Kolikhinol» dlya veterinarnogo primeneniya [Determination of acute and chronic toxicity of Coliquinol for veterinary use]. - Current issues for veterinary pharmacology, toxicology and pharmacy. - 2013. - pp. 646-649.

9. Ostrovskiy V. K. et al. Pokazateli krovi i leykotsitarnogo indeksa intoksikatsii v otsenke tyazhesti i opredelenii prognoza pri vospalitelnykh, gnoynykh i gnoyno-destruktivnykh zabolevaniyakh [Indices of blood and leukocyte index of intoxication in assessing the severity and determining the prognosis for inflammatory, purulent and purulent-destructive diseases]. - Klinicheskaya laboratornaya diagnostika. - 2006 (6). - pp. 128-132.

10. GOST 33215-2014. Rukovodstvo po soderzhaniyu i ukhodu za laboratornymi zhivotnymi. Pravila oborudovaniya pomeshcheniy i organizatsii protsedur [Guide to the care and maintenance of laboratory animals. Rules for equipment of premises and organization of procedures].

11. GOST 33216-2014. Rukovodstvo po soderzhaniyu i ukhodu za laboratornymi zhivotnymi. Pravila soderzhaniya i ukhoda za laboratornymi gryzunami i krolikami [Guide to the care and maintenance of laboratory animals. Rules for keeping and caring for laboratory rodents and rabbits].

12. Sidelnikova V. I. et al. Endogennaya intoksikatsiya i vospalenie: posledovatelnost reaktsiy i informativnost markerov (obzor) [Endogenous intoxication and inflammation: sequence of reactions and informativeness of markers (review)]. - Agricultural Biology. - 2015 (50 (2). - pp. 152-161.

13. Siplivyy V. A. et al. Ispolzovanie leykotsitarnykh indeksov dlya prognozirovaniya iskhoda peritonita [Use of leukocyte indices to predict the outcome of peritonitis]. - Klinicheskaya khirurgiya. - 2009 (9). - pp. 21-26.

14. Vereshchak N. A. et al. Farmako-toksikologicheskie issledovaniya kormovoy dobavki Lakto-Drayv na laboratornykh zhivotnykh [Pharmaco-toxicological studies of the feed additive Lacto-Drive on laboratory animals]. -Issues of legal regulation in veterinary medicine. - 2015 (2). - pp. 117-119.

Author affiliation:

Malkov Sergey V., Ph. D. in Veterinary Medicine, senior scientific researcher of the Department of ecology and non-infectious pathology of the Ural Federal Agrarian Scientific Research Centre (Ural Branch of the Russian Academy of Sciences); 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-912-2485082; e-mail: aibolit_2001@mail.ru.

Oparina Olga Yu., Ph. D. in Veterinary Medicine, senior scientific researcher of the Department of ecology and non-infectious pathology of the Ural Federal Agrarian Scientific Research Centre (Ural Branch of the Russian Academy of Sciences); 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-912-6011634; e-mail: olia91oparina@yandex.ru.

Belousov Aleksandr I., D. Sc. in Veterinary Medicine, senior scientific researcher of the Department of ecology and non-infectious pathology of the Ural Federal Agrarian Scientific Research Centre (Ural Branch of the Russian Academy of Sciences); 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-908-6390771; e-mail: white-knight@mail.ru.

Responsible for correspondence with the editorial board: Krasnoperov Aleksandr S., Ph. D. in Veterinary Medicine, senior scientific researcher of the Department of ecology and non-infectious pathology of the Ural Federal Agrarian Scientific Research Centre (Ural Branch of the Russian Academy of Sciences); 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-903-0833132; e-mail: marafon.86@list.ru.