Structure of opportunistic pathogenic microflora in various kinds of animal farms

DOI 10.33861/2071-8020-2019-5-18-21

Donnik I.M., Krivonogova A.S., Isaeva A.G., Musikhina N.B., Moiseeva K.V., Gordeev A.A.

Summary. The article describes the results of the research on structure of opportunistic pathogenic microflora in commercial dairy farms, pig-farming enterprises and poultry farms. The research was done on species composition of microbiota in wash-offs from mucosa, skin, and coat of animals, wash-offs from equipment, feed and drinking pans, fences, surfaces, tools; in samples of bedding, manure, drinking water, feed stuff, premix, and air. In commercial dairy farms research was done in maternity barns, calf-sheds, and departments for milking herd; in pig-farming enterprises - in departments for pregnant and farrowed sows, store pigs, nursery, and fattening; in poultry farms the samples from bedding and poultry dung and wash-off from cages, feeding and drinking pans and dung conveyor were taken; wash-offs from mucosa, feather and skin, from cloaca of laying hens of various age groups in egg-type farms and of broiler chickens of preslaughter age in meat-type farms. The research was done on frequency distribution of typical representatives of pathogenic and opportunistic pathogenic microflora in various kinds of animal enterprises. The analysis of interconnection between structure of opportunistic pathogenic microbiocenosis and profile of an enterprise, ecological status of agrobiocenosis and some definite technological factors was done. It was stated that there is interconnection between the level of technogenic contamination of environment and amount of P. aeruginosa, S. aureus and other representatives in structure of microflora in commercial dairy farms, and low variability of microbiological pattern and high homogeny of parameters of screening of microflora in pig-farming complexes, as compared to the parameters obtained in commercial dairy or poultry farms. The research was supported by a grant from the Russian Science Foundation (Project No. 18-16-00040).

Keywords: opportunistic pathogenic microflora, pathogenic microflora, structure of microflora, microbiocenosis, commercial dairy farm, pig-farming enterprise, poultry farm, technogenic pollution, microbiological screening, antibiotic resistance, S. aureus, P. aeruginosa, Ent. faecium.

References:

1. Donnik I.M., Loretz O.G., Barashkin M.I., Portnov V.S., Beykin Ya.B., Shkuratova I.A., Vereshchak N.A., Krivonogova A.S., Isaeva A.G. Fiziologicheskiye osobennosti zhivotnykh v rayonakh tekhnogennogo zagryazneniya [Physiological special characteristics of animals in areas of technogenic pollution]. - Veterinaria Kubani. - Krasnodar, 2013 (1). - pp. 21-22.

2. Ilchenko E.L., Smirnova Yu.A. Issledovaniye rezistentnosti bakteriy klassa staphylococcus k antibiotikam shirokogo spektra [Research on resistance of bacterium of staphylococcus to pluripotential antibiotics]. - 2018: 28-35.

3. Lartseva L.V., Obukhova O.V., Barmin A.N. Ekologicheskaya i biologicheskaya opasnost' rezistentnosti uslovno-patogennoy mikroflory k antibiotikam (obzor) [Ecological and biological danger of resistance of opportunistic pathogenic microflora to antibiotics (review)]. - 2015 (4 (4)). - pp. 47-52.

4. Sbornik materialov konferentsiy [Conference proceedings]. - Moscow, 2015.

5. Shevchenko A.A., Chernykh O.Yu., Samuylenko A.Ya., Donnik I.M., Grin S.A., Shevchenko L.V., Shevkoplyas V.N., Koshchayev A.G., Krivonogova A.S., Isaeva A.G., Krasochko P.A., Motuzko N.S. Diagnostika infektsionnykh bolezney selskokhozyaystvennykh zhivotnykh: bakterialnyye zabolevaniya [Diagnosis of infectious diseases of agricultural animals: bacterial diseases]. - Krasnodar, 2018.

6. Chebotar I.V., Bocharova Yu.A., Mayanskiy N.A. Mekhanizmy rezistentnosti Pseudomonas aeruginosa k antibiotikam i ikh regulyatsiya [Mechanisms of Pseudomonas aeruginosa resistance to antibiotics and their regulation]. - 2017: 308-319.

7-16. Vide supra.

Author affiliation:

Donnik Irina M., Vice-president of the Russian Academy of Sciences, head of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-3713363; e-mail: ktqrjp7@yandex.ru.

Krivonogova Anna S., D.Sc. in Biology, docent, leading scientific researcher of the Department of monitoring and forecasting of infectious diseases of the Ural Federal Agrarian Scientific Research Centre of the Ural Branch of the Russian Academy of Sciences; 112 а, Belinskogo st., Ekaterinburg, 620142; phone: 8-982-6512934; e-mail: tel-89826512934@yandex.ru.

Musikhina Nina B., assistant of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-905-8092603; e-mail: musikhina83@mail.ru.

Moiseeva Ksenia V., assistant of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-953-0475887; e-mail: moiseeva456@yandex.ru.

Gordeev Aleksey A., post-graduate student of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-2214110.

Responsible for correspondence with the editorial board: Isaeva Albina G., D.Sc. in Biology, docent, professor of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-2951774; e-mail: isaeva.05@bk.ru.