Biochemical markers bone formation and inflammation in dogs during Ilizarov tibial lengthening

UDC 612.11-002:599.742.1-092.9:616.718.5/.6-001.5-089.227.84

Tushina N.V., Talashova I.A., Kononovich N.A.

Summary. Concentration of total protein, protein fractions, C-reactive protein, sialic acids, activity of alkaline and acidic phosphatase were studied in blood serum of mongrel dogs, which underwent tibial lengthening by the method of transosseous distraction osteosynthesis. C-reactive protein, sialic acids were studied, the activity of alkaline and acidic phosphatase. The animals were divided into 3 groups: in the 1st group - the dogs after closed flexion osteoclasia underwent Ilizarov tibial lengthening at the rate 1 mm/day subdivided into 4 movements manually, in the 2nd group - the dogs underwent Ilizarov tibial lengthening at the rate 1.5 mm/ day subdivided into 6 movements manually, in the 3rd - the dogs underwent Ilizarov tibial lengthening at the rate 3 mm/day subdivided into 120 movements in automatic mode. It is revealed that in the animals of group 1 significant activity osteoliticescoy phase (increase alkaline phosphatase) were observed in the second half of distraction; in group 2 - in the first half (first 15 days) fixation; in group 3, the activity of bone formation was significantly suppressed, as in the second half of distraction (10-20 days) and during the commit phase. In experimental animals of all three groups we discovered increase of the level of sialic acids, total protein and globulin fractions secondary to hypoalbuminemia. Based on the results we can conclude: inflammatory reaction is a mandatory component of distraction, and its intensity can determine further character and outcome of reparative processes.

Keywords: locomotor system, distraction osteosynthesis, distraction osteogenesis, tibia bone lengthening, flexion osteoclasis, Ilizarov method, blood biochemistry, bone formation markers, osteosynthetic phase, inflammatory response.

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Author affiliation:

Talashova Irina А., Ph.D. in Biology, senior researcher of the laboratory of biochemistry of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: i_talashova@mail.ru.

Kononovich Natalya A., Ph.D. in Veterinary Medicine, leading researcher of the laboratory for correction of deformities and lengthening of the limbs, veterinarian of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: n.a.kononovich@mail.ru.

Responsible for correspondence with the editorial board: Tushina Natalya V., Ph.D. in Biology, researcher of the laboratory of biochemistry of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: ntushina76@mail.ru.