UDC 612.11-002:599.742.1-092.9:616.718.5/.6-001.5-089.227.84
Tushina N.V., Talashova I.A., Kononovich N.A.
Summary. Concentration of total protein, protein fractions, C-reactive protein, sialic acids, activity of alkaline and acidic phosphatase were studied in blood serum of mongrel dogs, which underwent tibial lengthening by the method of transosseous distraction osteosynthesis. C-reactive protein, sialic acids were studied, the activity of alkaline and acidic phosphatase. The animals were divided into 3 groups: in the 1st group - the dogs after closed flexion osteoclasia underwent Ilizarov tibial lengthening at the rate 1 mm/day subdivided into 4 movements manually, in the 2nd group - the dogs underwent Ilizarov tibial lengthening at the rate 1.5 mm/ day subdivided into 6 movements manually, in the 3rd - the dogs underwent Ilizarov tibial lengthening at the rate 3 mm/day subdivided into 120 movements in automatic mode. It is revealed that in the animals of group 1 significant activity osteoliticescoy phase (increase alkaline phosphatase) were observed in the second half of distraction; in group 2 - in the first half (first 15 days) fixation; in group 3, the activity of bone formation was significantly suppressed, as in the second half of distraction (10-20 days) and during the commit phase. In experimental animals of all three groups we discovered increase of the level of sialic acids, total protein and globulin fractions secondary to hypoalbuminemia. Based on the results we can conclude: inflammatory reaction is a mandatory component of distraction, and its intensity can determine further character and outcome of reparative processes.
Keywords: locomotor system, distraction osteosynthesis, distraction osteogenesis, tibia bone lengthening, flexion osteoclasis, Ilizarov method, blood biochemistry, bone formation markers, osteosynthetic phase, inflammatory response.
References:
1. Erofeev S.A., Luneva S.N., Stogov M.V., Tushina N.V. Biokhimicheskie issledovaniya syvorotki krovi i skeletnykh myshts pri udlinenii goleni apparatom Ilizarova s tempom distraktsii 3 mm v sutki v avtomaticheskom rezhime [Biochemical studies of blood serum and skeletal muscles during tibial lengthening with the rate of 3 mm per day in automatic mode]. - Vestnik novykh meditsinskikh tekhnologiy. - Tula, 2005 (3, 4). - pp. 89-91.
2. Gorbach Ye.N., Stepanov M.A. Osobennosti morfogeneza kostnoy tkani pri udlinenii goleni metodom chreskostnogo distraktsionnogo osteosinteza pri povyshennom sutochnom tempe [Features of morphogenesis of bone tissue in tibial lengthening by distraction osteosynthesis at higher daily rate]. - Morphology. - Saint-Petersburg, 2015 (147 (2)). - ðð. 69-74.
3. Kamyshnikov V.S. Spravochnik po kliniko-biokhimicheskoy laboratornoy diagnostike [Handbook on clinical-biochemical laboratory diagnostics]. - Minsk, 2000. - 463 p.
4. Kirsanov K.P., Krasnov V.V., Timofeev V.N. Tehnologiya lecheniya povrezhdeniy taza u melkikh domashnikh zhivotnykh metodom chreskostnogo osteosinteza [Therapeutic technology of pelvis injuries in small animals by transosseous osteosynthesis]. - Veterinarnaya patologiya. - Moscow, 2006 (2). - pp. 57-63.
5. Timofeyev S.V., Fillipov Yu.I., Bakhtinov V.A., Petrovskaya N.V., Kononovich N.A. Lechenie otkrytykh diafizarnykh perelomov kostey goleni u koshek [Therapy of open diaphyseal fractures of the tibia in cats]. - Veterinariya. - Moscow, 2006 (2). - pp. 61-62.
6. Nevedrov A.V., Manuilov K.V. Operativnoe lechenie lozhnykh sustavov kostey goleni u sobak metodom chreskostnogo kompressionno-distraktsionnogo osteosinteza apparatom G.A. Ilizarova [Surgical treatment of false joints of tibial bones in dogs by the method of transosseous compression-distraction osteosynthesis by Ilizarov apparatus]. - Veterinariya. - Moscow, 2004 (5). - pp. 56-57.
7. Yagodnikov S.Ya., Kozhushko P.S., Kuleshova O.A., Kuleshova Ya.A. Oslozhneniya u sobak karlikovykh porod pri lechenii perelomov kostey predplechya [Complications in the therapy of àntebrachial fractures in dogs of small breeds]. - Logos Press. - Moscow, 2014 (1). - pp. 6-10.
8. Stogov M.V., Kuznetsova L.S., Luneva S.N., Erofeev S. A. Osobennosti energeticheskogo metabolizma skeletnykh myshts sobak v usloviyakh udlineniya goleni po Ilizarovu [Energy metabolism in skeletal muscles of dogs with tibial lengthening by Ilizarov method]. - RAMN. - 2002 (6). - pp. 176-179.
9. Kolesnikova E.S., Stogov M.V., Novikov K.I., Veliev Z.S., Tushina N.V. Otsenka osteoreparatsii u patsientov v khode kosmeticheskoy korrektsii dliny nizhney konechnosti po metodu Ilizarova [Osteoreparation assessment in patients during lower limb length cosmetic correction according to Ilizarov method]. - Geniy ortopedii. - Kurgan, 2013 (2). - pp. 98-101.
10. Popkov A.V., Popkov D.A. Distraktsionnyy osteosintez - perspektivy razvitiya [Distraction osteosynthesis - developmental outlooks]. - Geniy ortopedii. - Kurgan, 2006 (4). - pp. 30-37.
11. Silanteva T.A., Krasnov V.V., Dobyychina N.A. Reparativnaya regeneratsiya i adaptivnaya perestroyka tkaney nizhney chelyusti pri lechenii ee mnozhestvennoy travmy metodom chreskostnogo osteosinteza (eksperimentalno-morfologicheskoe issledovanie) [Reparative regeneration and adaptive restructuring of the mandible tissues in the therapy of multiple trauma by its transosseous osteosynthesis (experimentally-morphological research)]. - Stomatologiya. - Moscow, 2012 (91 (4)). - pp. 7-10.
12. Shakirova F.V. Metod chreskostnogo vneochagovogo osteosinteza sterzhnevym apparatom naruzhney fiksatsii u sobak [Transosseous extrafocal osteosynthesis method by apparatus of external fixation in dogs]. - Lan. - Saint-Petersburg, 2009 (1). - pp. 19-21.
13. Shevchenko O.P, Dolgov V.V., Olefirenko G.A. Elektroforez v klinicheskoy laboratorii [Electrophoresis in the clinical laboratory]. - Reafarm. - Moscow, 2006. - 160 p.
14-15. Vide supra.
Author affiliation:
Talashova Irina À., Ph.D. in Biology, senior researcher of the laboratory of biochemistry of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: i_talashova@mail.ru.
Kononovich Natalya A., Ph.D. in Veterinary Medicine, leading researcher of the laboratory for correction of deformities and lengthening of the limbs, veterinarian of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: n.a.kononovich@mail.ru.
Responsible for correspondence with the editorial board: Tushina Natalya V., Ph.D. in Biology, researcher of the laboratory of biochemistry of the Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics of the Ministry of health of the Russian Federation; 6, M. Ulyanova st., Kurgan, 640014; e-mail: ntushina76@mail.ru.
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