Sensitivity assessment of a newly developed set of reagents for the diagnosis of fish aeromonosis by ‘real-time’ PCR

УДК 619:639.3.09
DOI 10.33861/2071-8020-2024-4-31-34

Tokareva S. B., Kudinov P. V., Sekhina O. V., Romanova N. N., Shchelkunova Yu. P., Baryshnikova E. I., Kaloshkina I. M.

Summary. Data on the results of assessing the sensitivity of the set of reagents for the diagnosis of aeromonosis of fish by PCR in ‘real-time’ mode in comparison with the classical bacteriological analysis on nutrient media in the study of pathological material selected from experimentally infected with Aeromonas spp. bacteria are presented in the article. In the early stages of the disease, before the development of clinical signs, diagnosis by PCR-RV using the developed set of reagents was inferior in sensitivity to the classical method of bacteriological analysis on nutrient media. However, at the peak of the disease, the sensitivity of the PCR-RV method was higher in comparison with the classical method.

Keywords: fish, bacterial diseases, fish aeromonosis, diagnostics, polymerase-chain reaction, real-time PCR.

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Author affiliation:

Tokareva Svetlana B., Chief Specialist of the Ichthyopathology Laboratory of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 a, Rybnoe vllg., Moscow region, 141821; e-mail: tokareva@vniiprh.vniro.ru.

Kudinov Pavel V., Chief Specialist of the Ichthyopathology Laboratory of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 a, Rybnoe vllg., Moscow region, 141821; e-mail: kudinov@vniiprh.vniro.ru.

Sekhina Olga V., Specialist of the Ichthyopathology Laboratory of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 a, Rybnoe vllg., Moscow region, 141821; e-mail: sekhina@vniiprh.vniro.ru.

Shchelkunova Yulia P., Ph. D. in Biology, Deputy Head of the Ichthyopathology Laboratory of, Candidate of Biological Sciences, Branch for Freshwater Fisheries of the Federal State of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 a, Rybnoe vllg., Moscow region, 141821; e-mail: schelkunova@vniiprh.vniro.ru.

Baryshnikova Elena I., Ph. D. in Biology, Senior Specialist in the development of new products of NextBio LLC; 2-8, Polimernaya st., Moscow, 111394; e-mail: elena-baryshnikova85@yandex.ru.

Kaloshkina Inna M., Ph. D. in Veterinary Medicine, head of the department of antiparasitic, veterinary and sanitary measures of the Krasnodar regional station of fighting against animal diseases; 15/1, Kalinina st., Krasnodar, 350004; phone: 8-861-2216091; e-mail: beretarinna@gmail.com.

Responsible for correspondence with the editorial board: Romanova Natalia N., Ph. D. in Biology, Docent, Head of the Ichthyopathology Laboratory of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 a, Rybnoe vllg., Moscow region, 141821; phone: 8-495-1086856 (+132); e-mail: romanova@vniiprh.vniro.ru.