Standardization of quality indicators and toxicological evaluation of the drug revinorm intended for the treatment of acute heat stress in animals

УДК 619:615.4
DOI 10.33861/2071-8020-2023-4-18-21

Kuzminova E.V., Sampiev A.M., Semenenko M.P., Abramov A.A.

Summary. One of the most serious problems of modern times is climate change, which directly affects the agricultural sector, including livestock farming. Therefore, heat stress in productive animals is one of the most common and economically significant problems in the industry, and the development of effective drugs to combat this pathology is a current area of veterinary pharmacology. Authors standardized the quality and assessed the safety of the drug revinorm, intended for the treatment of acute heat stress in animals. The standardization of drug quality indicators was carried out in accordance with the requirements of the general pharmacopoeial articles of the State Pharmacopoeia of the Russian Federation, XIV edition. The acute toxicity of revinorm was studied on nonlinear laboratory rats using the intragastric administration at a dose of 7000 mg/kg of body weight. Chronic toxicity was assessed in three groups of rats: two experimental and one control (n=10). For three months, the animals of the experimental groups individually received the drug on an empty stomach once a day in the form of boluses in doses of 1/10 and 1/20 of the maximum administered dose in the acute experiment; control group received boluses without active substance in equal volume and in the same dosage regimen. Based on the results of the work, quality indicators were standardized for the drug revinorm. Based on toxicological studies, it was determined that according to GOST 12.1.007-76 «Harmful substances», revinorm belongs to hazard class IV (low-hazard substances), and with prolonged oral use it is harmless to warm-blooded animals.

Keywords: drug revinorm, animal husbandry, productive animals, heat stress, therapy, quality indicators, toxicological assessment, veterinary pharmacology, climate change, oral administration.

References:

1. Biryukova N. P., Rusakov S. V., Napalkova V. V. Obshchie printsipy doklinicheskoy otsenki bezopasnosti farmakologicheskikh lekarstvennykh sredstv dlya veterinarnogo primeneniya [General principles of preclinical assessment of the safety of pharmacological drugs for veterinary use]. -Veterinarnyy vrach. - 2018 (1). - pp. 3-9.

2. Belousov A. I. et al. Vliyanie teplovogo stressa na korov v sukhostoynyy i poslerodovoy period [Influence of heat stress on cows during the dry and postpartum period]. - 2022 (3 (64). - pp. 93-101.

3. Zalikhanov M. Ch. et al. Vozmozhnyye posledstviya izmeneniya klimata na Yuge evropeyskoy territorii Rossii [Possible consequences of climate change in the south of the European territory of Russia]. - Advances in modern natural science. - 2021 (12). - pp. 107-113.

4. Gladilshchikova A. A., Dmitrieva T. M. Zavershenie shestogo tsikla raboty Mezhpravitelstvennoy gruppy ekspertov po izmeneniyu klimata [Completion of the sixth cycle of work of the Intergovernmental Panel on Climate Change]. - Fundamental and Applied Climatology. - 2023 (9 (1). -pp. 5-12.

5. Gosudarstvennaya Farmakopeya Rossiyskoy Federatsii XIV izdaniya [State Pharmacopoeia of the Russian Federation XIV edition]. - https:// pharmacopoeia.regmed.ru/pharmacopoeia/izdanie-14/

6. Dorozhkin V. I., Biryukova N. P., Bakhmutova T. V. Sovremennye trebovaniya k izucheniyu obshchetoksicheskogo deystviya farmakolo-gicheskikh veshchestv [Modern requirements for the study of the general toxic effect of pharmacological substances]. - 2019.

7. Kovaleva G. P. et al. Vliyanie teplovogo stressa na vosproizvoditelnuyu sposobnost molochnykh korov i sposob ee korrektsii [Influence of heat stress on the reproductive ability of dairy cows and the method of its correction]. -Agricultural Journal. - 2022 (2 (15). - pp. 58-65.

8. Bardin M. Yu. et al. Obzor sovremennogo sostoyaniya i izmeneniy klimata Rossiyskoy Federatsii [Review of the current state and climate changes in the Russian Federation]. - 2020 (3 (163). - pp. 69-77.

9. Papikyan K. A., Kornitskaya O. V. Izmenenie klimata - ugroza ekologicheskoy bezopasnosti [Climate change is a threat to environmental safety]. - 2023.

10. Paptsov A. G. et al. Globalnaya prodovolstvennaya bezopasnost v usloviyakh klimaticheskikh izmeneniy [Global food security under climate change]. - RAS. - 2018: 132.

11. Rakhmatullin E. K. et al. Izuchenie toksichnosti veterinarnykh preparatov na doklinicheskom etape [Study of the toxicity of veterinary drugs at the preclinical stage]. - Bulletin of Russian Agricultural Science. -2019 (5). - pp. 61-64.

12. Kuzminova E. V. et al. Sostoyanie biokhimicheskogo profilya krovi i urovnya endogennoy intoksikatsii u korov s gepatopatiyami v usloviyakh teplovogo stressa [State of the biochemical blood profile and the level of endogenous intoxication in cows with hepatopathy under heat stress]. -Veterinary Science Today. - 2022 (11 (2). - pp. 135-141.

13. Rud E. N. et al. Sostoyanie perekisnogo okisleniya lipidov v organizme molochnykh korov pri teplovom stresse [State of lipid peroxidation in the body of dairy cows under heat stress]. - Proceedings of the Kuban State Agrarian University. - 2021 (92). - pp. 238-244.

Author affiliation:

Kuzminova Elena V., D.Sc. in Veterinary Medicine, docent, chief scientific researcher of the Department of Pharmacology of the Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine; 1, 1-ya Liniya st., Krasnodar, 350004; phone: 8-918-4198369; e-mail: niva1430@mail.ru.

Sampiev Abdulmutalip M., D. Sc. in Pharmaceuticals, professor, leading scientific researcher of the Department of Pharmacology of the Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine; 1, 1-ya Liniya st., Krasnodar, 350004; phone: 8-928-0375500; е-mail: sampiev_abdul@mail.ru.

Semenenko Marina P., D.Sc. in Veterinary Medicine, docent, Head of the Department of Pharmacology of the Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine; 1, 1-ya Liniya st., Krasnodar, 350004; phone: 8-918-4612663; е-mail: sever291@mail.ru.

Responsible for correspondence with the editorial board: Abramov Andrey A., Ph.D. in Veterinary Medicine, senior scientific researcher of the Department of Pharmacology of the Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine; 1, 1-ya Liniya st., Krasnodar, 350004; phone: 8-902-4061527; e-mail: abramov1527@mail.ru.