Problem of antiviral vaccines' contamination in the world and in Russia

DOI 10.33861/2071-8020-2019-3-3-6

Chernykh O.Yu., Mishchenko A.V., Mishchenko V.A., Krivonos R.A., Drobin Yu.D., Lysenko A.A.

Summary. Presented literature data indicate the serious threat of live virusvaccines contaminated with the causative agent of viral diarrhea virus, border disease of sheep and mycoplasma to livestock of almost all countries in the world. In cows vaccinated with the indicated vaccines abortions, stillbirths and birth of unviable calves are recorded. The literature data on the facts of contamination by cytopathogenic and non-cytopathogenic biotypes of viral diarrhea virus 13 genotypes, fetal blood serum of large horned cattle used as one of the main components of the nutrient medium for cell culture cultivation is given. The results of studies on the contamination of transplantable cell culture lines by viruses such as viral diarrhea virus, parvovirus, ceneca virus, and pathogen of sheep border infection and mycoplasma are summarized. In the study of 117 cell series and 35 samples of fetal bovine serum for contamination of bovine viral diarrhea with the virus, viral diarrhea virus was detected in 25% of cell samples and in 45% of bovine fetal serum. Facts of trypsin contamination with parvovirus were established. Mycoplasma were detected in 20% of samples during the study of 69 cell cultures. Authors noted that large number of series of fetal calves' serum were contaminated by viral diarrhea virus in Russia. Authors confirmed the data that cell cultures used in the production of live antiviral vaccines should not be used for more than 20 passages, and each passage should be checked for the presence of contaminating viruses. The use of Fetal Clone (serum substitute) as a component of the growth medium for growing transplantable cell lines allows to obtain viral material with high antigenic and immunogenic activity, free from mycoplasma and virus contaminants.

Key words: pregnant cows, bovine viral diarrhea virus 1-3 genotypes, cytopathogenic and non-cytopathogenic biotypes, parvoviruses, mycoplasma, bacteria, fungi, cell cultures, blood serum, harmlessness, virus-vaccine.

References:

1. Uryvayev L.V., Ionova K.S., Dedova A.V. et al. Analiz kontaminatsii kletochnykh kultur pestivirusom BVDV i mikoplazmami [Analysis of cell culture contamination with BVDV pestivirus and mycoplasma]. - Voprosy virusologii. - Moscow, 2012 (57 (5)). - pp. 15-21.

2. Anoyatbekova A.M., Alekseyenkova S.V., Yurov K.P. Molekulyarno-evolyutsionnyy geneticheskiy analiz virusa pogranichnoy bolezni, vyyavlennogo u ovets v Tadzhikistane [Molecular evolutionary genetic analysis of the virus of borderline disease detected in sheep in Tajikistan]. - Veterinariya. - Moscow, 2017 (10). - pp. 23-26.

3. Yurov G.K., Alekseyenkova S.V., Dias Khimenes K. et al. Antigennyye svoystva netsitopatogennykh shtammov virusa diarei - bolezni slizistykh krupnogo rogatogo skota [Antigenic properties of non-cytopathogenic virus strains diarrhea - disease of mucous membranes of large horned cattle]. - 2013: 24-26.

4. Lugovtsev V.Yu., Vishnyakov I.F. Pogranichnaya bolezn ovets (obzor inostrannoy literatury) [Sheep border disease (review of foreign literature)]. - Vestnik RASKhN. - Moscow, 1998 (1). - pp. 79-82.

5. Lugovtsev V.Yu., Vishnyakov I.F. Pogranichnaya bolezn ovets (obzor inostrannoy literatury) [Sheep border disease (review of foreign literature)]. - Vestnik RASKhN. - Moscow, 1998 (2). - pp. 53-58.

6. Lugovtsev V.Yu., Vishnyakov I.F. Pogranichnaya bolezn ovets (obzor inostrannoy literatury) [Sheep border disease (review of foreign literature)]. - Vestnik RASKhN. - Moscow, 1998 (3). - pp. 64-67.

7. Mishchenko A.V., Semakina V.P., Mishchenko V.A. et al. Massovyye bolezni sviney s vezikulyarnym sindromom [Massive diseases of pigs with vesicular syndrome]. - Veterinariya. - Moscow, 2016 (7). - pp. 12-19.

8. Orlyankin B.G., Rayev S.A., Aliper T.I. et al. Novaya massovaya bolezn sviney - senekavirusnaya infektsiya [New swine mass disease - seneca virus infection]. - Svinovodstvo. - Moscow, 2016, 7(16). - pp. 46-47.

9. Uryvayev L., Dedova A., Dedova L. et al. O kontaminatsii kletochnykh kultur virusom diarei - bolezni slizistykh obolochek krupnogo rogatogo skota (BVDV) [On contamination of cell cultures with diarrhea virus - disease of mucous membranes in large horned cattle (BVDV)]. - 2012: 88-93.

10. Mishchenko V.A. et al. Optimizatsiya usloviy kultivirovaniya virusov krupnogo rogatogo skota v perevivayemykh kulturakh kletok [Optimization of conditions for cultivation of bovine viruses in transplantable cell cultures]. - Veterinariya. - Moscow, 2014 (2). - pp. 60-63.

11. Kononova S.V. et al. Optimizatsiya reaktsii mikroneytralizatsii dlya opredeleniya antitel k virusu virusnoy diarei krupnogo rogatogo skota [Optimization of microneutralization reaction for determination of antibodies to bovine viral diarrhea virus]. - Veterinariya. - Moscow, 2015 (9). - pp. 58-62.

12. Mishchenko V.A., Gusev A.A., Zakharov V.M. et al. Osobennosti parvovirusnoy infektsii krupnogo rogatogo skota [Features of parvoviral infection of large horned cattle]. - Veterinariya. - Moscow, 2000 (5). - pp. 19-21.

13. Mironova L.L. et al. Otechestvennyye linii perevivayemykh kletok pochek telenka - substrat dlya biotekhnologii [Domestic lines of transplanted calf kidney cells - substrate for biotechnology]. - 1988: 25-31.

14. Chernykh O.Yu. et al. Problema virusnoy diarei krupnogo rogatogo skota [Problem of viral diarrhea in large horned cattle]. - Trudy KubGAU. - Krasnodar, 2016 (1 (58)). - pp. 194-198.

15. Prigodnost kletochnykh substratov dlya proizvodstva biologicheskikh preparatov [Suitability of cellular substrates for production of biological products]. - Geneva, 1988.

16. Alekseyenkova S.V., Yurov K.P., Galnbek T.V. et al. Proverka kletochnykh kultur na kontaminatsiyu virusom diarei krupnogo rogatogo skota - neobkhodimoye usloviye proizvodstva biologicheskikh preparatov [Checking cell cultures for contamination of cattle diarrhea with the virus is a prerequisite for the production of biological products]. - 2012: 15-18.

17. Sergeyev V.A. et al. Virusy i virusnyye vaktsiny [Viruses and virus vaccines]. - Biblionika. - Moscow, 2007. - pp. 73-78.

18. Chernykh O.Yu., Mishchenko A.V., Mishchenko V.A. et al. Spetsificheskaya profilaktika nodulyarnogo dermatita krupnogo rogatogo skota [Specific prevention of bovine nodular dermatitis]. - Veterinariya Kubani. - Krasnodar, 2016 (3). - pp. 3-5.

19. Trebovaniya k perevivayemym liniyam kletok, ispolzuyemykh dlya proizvodstva biologicheskikh preparatov [Requirements for transplantable cell lines used for production of biological products]. - 1988: 85-88.

20. Shulpin M.I., Ayanot P.K., Mishchenko V.A. Indikatsiya virusa diarei krupnogo rogatogo skota, genotipirovaniye i filogeneticheskiy analiz izolyatov, vydelennykh na territorii Rossiyskoy Federatsii [Indication of bovine diarrhea virus, genotyping and phylogenetic analysis of isolates in the Russian Federation]. - Voprosy virusologii. - Moscow, 2003 (5). - pp. 41-45.

21. Yurov K.P., Anoyatbekova A.M., Alekseyenkova S.V. Novyy pestivirus-HoBi virus - kontaminant vaktsin protiv chumy melkikh zhvachnykh [New pestivirus-HoBi virus - contaminant of small ruminant plague vaccines]. - Veterinariya. - Moscow, 2016 (10). - pp.8-11.

22. Chernykh O.Yu. et al. Ekologicheskiye osobennosti vozbuditelya virusnoy diarei krupnogo rogatogo skota 1 i 2 genotipov [Ecological features of causative agent of viral diarrhea of large horned cattle of 1st and 2nd genotypes]. - Trudy KubGAU. - Krasnodar, 2016 (61). - pp. 149-156.

23-51. Vide supra.

Author affiliation:

Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin regional veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; phone: 8-918-4956659; e-mail: gukkvl50@kubanvet.ru.

Mishchenko Aleksey V., Ph.D. in Veterinary Medicine, Senior scientific researcher of the information analysis center of the Federal Centre of Animal Health; mcrd. Yurjevets, Vladimir, Russia, 600901; phone: 8-4922-261551; e-mail: a.mischenko@mcx.ru.

Mishchenko Vladimir A., D.Sc. in Veterinary Medicine, professor, chief scientific researcher of the Laboratory of the prevention of diseases of pigs and horned cattle of the Federal Centre of Animal Health; mcrd. Yurjevets, Vladimir, Russia, 600901; phone: 8-4922-261551; e-mail: mishenko@arriah.ru.

Krivonos Roman A., Ph.D. in Veterinary Medicine, head of the Veterinary Department of Krasnodar region; 36, Rashpilevskaya st., Krasnodar, 350000; phone: 8-861-2622869; e-mail: uv@krasnodar.ru.

Drobin Yury D., Ph.D. in Agriculture, Director of the North Caucasian Zonal Scientific-Research Veterinary Institute; Rostovskoe hgw., Novocherkassk, Rostov area, 346421; phone: 8-8635-266270; e-mail: skznivi@novoch.ru.

Responsible for correspondence with the editorial board: Lysenko Aleksandr A., D.Sc. in Veterinary Medicine, professor, professor of the department of therapy and pharmacology of the Kuban State Agrarian University named after I.T. Trubilin; 7, 29, Rozhdestvenskaya emb., Krasnodar, 350089; phone: 8-9615075415; e-mail: vet.kubgau@mail.ru.