Parameters of suspension cultivation of cell culture BHK 21/13-13 in biotechnological production scale

UDC 619:578:616.98:578.828.11

Kryukova E.N., Samuylenko A.Ya., Melnik R.N., Elnikov V.V., Krasutkin S.N., Litenkova I.Yu., Melnik N.V., Borovoy V.N., Dresvyannikova S.G.

Summary. Suspension cultures are the most promising in solving practical problems related to the obtaining of viral and cellular materials. Cells in suspension at favorable conditions give a higher yield than in stationary conditions. Scientific data for the study of the suspension cultivation of cell culture BHK-21/13-13 on a scale of biotechnological production, adaptation to the slurry process of growing and using it in the manufacture of FMD and rabies vaccines is presented the article. Technological stepwise scheme for the production of BHK-21/13-13 cells bank is shown. Cultivation parameters in the KS-40 in a volume of 30 liters, growing cells in 200-600 liter reactors in industrial production of viral vaccines are considered. Main indices of technological mode of BHK-21/13-13 cell culture cultivation under production conditions are described in the article. Authors improved technology for low-cost effective universal vaccine for large and small horned cattle, dogs and cats that are competitive to best modern foreign analogues according to immunogenicity and stability. Tests for studying the initial seeding cell concentration and mixing modes were carried out in order to establish the optimum mode of cell culture and virus in suspension. Technology developed by the authors will meet the needs of Agriculture of the Russian Federation and CIS countries in the effective preparations and make a significant contribution to the improvement of epizootic situation according to foot-and-mouth disease and rabies, outline ways to improve technologies with the transfer to innovative manufacturing techniques of FMD, rabies vaccines, using foot-and-mouth disease and rabies viruses, BHK 21/13-13 cells cultivated in suspension.

Keywords: cell culture, suspension, nutrient medium, suspension cultivation, transplantable cell subline, passage, filters, reactors, technological scheme of production, aeration.

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Author affiliation:

Kryukova Elena N., graduate of the All-Russian scientific-research and technological institute of biological production; Biocombinat, Moscow region, 141142; phone: 8(985)3948012; e-mail: krukovaen@biocombinat.ru.

Samuylenko Anatoliy Ya., D.Sc. in Veterinary Medicine, Academician of the RAS, director of the All-Russian scientific-research and technological institute of biological production; Biocombinat, Moscow region, 141142; phone: 8(910)4253914; e-mail: vnitibp@mail.ru.

Elnikov Vasiliy V., Ph.D. in Biology, chief technologist of the Shchelkovo Biokombinat; Biocombinat, Moscow region, 141142; phone: 8(983)948035; e-mail: velnikov@yandex.ru.

Krasutkin Sergey N., Ph.D. in Biology, antiviral production advisor of the Shchelkovo Biokombinat; Biocombinat, Moscow region, 141142; phone: 8(985)3948031; e-mail: krasotkin@biocombinat.ru.

Litenkova Irina Yu., Ph.D. in Veterinary Medicine, chief technologist of the scientific-research work of the Shchelkovo Biokombinat; Biocombinat, Moscow region, 141142; phone: 8(985)3948034; e-mail: litenkova2012@mail.ru.

Borovoy Vladimir N., Ph.D. in Veterinary Medicine, vice-director of the Veterinary Department of the Ministry of Agriculture of the Russian Federation; 11/1, Sadovo-Spasskaia st., Moscow, 107139; phone: 8(916)2369350; e-mail: borovoi-vn@mail.ru.

Melnik Nikolay V., D.Sc. in Veterinary Medicine, professor, president of the National Association of veterinary-biological industry (Vetbioprom); 11/1, Sadovo-Spasskaya st., Moscow, 107139; phone: 8(905)5450346; e-mail: a-vbp@mail.ru.

Dresvyannikova Svetlana G., Ph.D. in Veterinary Medicine, Head of the Krasnodar regional station of fighting against animal diseases; 15/1, Kalinina st., Krasnodar, 350004; phone: 8 (861) 221-63-60; e-mail: kraivet.dsg@mail.ru.

Responsible for correspondence with the editorial board: Melnik Roman N., Ph.D. in Veterinary Medicine, head of the department of the All-Russian scientific-research and technological institute of biological production; Biocombinat, Moscow region, 141142; phone: 8(926)8510696; e-mail: mromanos@mail.ru.