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Diagnostic evaluation of PCR and RDIF using samples from field outbreaks of African swine fever

UDC619:616.98:076

DIAGNOSTIC EVALUATION OF PCR AND RDIF USING SAMPLES FROM FIELD OUTBREAKS OF AFRICAN SWINE FEVER

Kurinnov V.V., Vasilev A.P., Belyanin S.A., Strizhakova O.M., Nogina I.V.,
Sidlik M.V., Gazaev I.Kh., Mironova L.P., Alikova G.A., Tsybanov S.Zh., Dzhailidi G.A., Chernykh O.Yu.

Summary. Statistical parameters of diagnostic polymerase chain reaction and direct immunofluorescence reaction based on the use of complex project stratified sample test population of primarily infected domestic pigs and wild boars from field cases or outbreaks of African swine fever are presented in the article. The results of PCR testing of unpaired and paired samples tests of parallel evaluation of MsNemar’s test did not provide significant differences between PCR and RDF. This indicates chance of false-negative results of PCR with unpaired testing, i.e. the uncertainty caused by the effect of an empirical factor in project selection. In the study of paired samples from dead pigs were from ASF albeit minor, but the differences of sensitivity of PCR and RDIF were 2.64 ( 0,18-4,4 )% (P = 0.01), which is statistically even more pronounced when testing of random samplies of hunted boars - 6.99 ( 1,6-10,5 )% (P = 0.01). PPR values in the parallel system "PCR RDIF" were significantly higher for the confirmation of the ASF, from 95,7 (86,0-98,4) 10% in prevalence ( 3-24 % ) in large herds to 99.2 (98.5-99.5) and 99.6 % (99,3-99,7)% - in the medium and small (and 53 %, respectively). Almost parallel system PCR-RDIF has good reliability and reproducibility to confirm clinical cases of ASF in any herds in domestic pigs and wild boars without confirmation of positive results of the reference method.

Keywords: African swine fever outbreak, laboratory diagnostics, sample, tests, polymerase chain reaction, direct immunofluorescence reaction, parallel testing, predictive positive results, prognostic negative results.

References:

1. Belyanin S.A. Dinamika rasprostraneniya i monitoring epizooticheskogo protsessa afrikanskoy chumy sviney v Rossiyskoy Federatsii N° 3 / 2014 [Dynamics of distribution and monitoring of the epizootic process of African swine fever in the Russian Federation]. – Pokrov, 2013. – p.27.

2. Vishnyakov I.F., Mitin N.I., Karpov G.M., Kurinnov V.V., Yashin A.T. Diagnostika i differentsialnaya diagnostika afrikanskoy i klassicheskoy chumy sviney [Diagnosis and differential diagnosis of African and classical swine fever]. – Veterinariya. – Moscow, 1991 (4). –pp.28-30.

3. Bakulov I.A., Vishnyakov I.F., Balabanov V.A., Kurinnov V.V. Svinyi. Metody laboratornoy diagnostiki afrikanskoy chumy sviney [Pigs. Methods of laboratory diagnosis of African swine pigs]. – Moscow, 1990.

4. Gazaev I.Kh., Elsukova A.A., Sindryakova I.P., Tsybanov S.Zh., Kolbasov D.V. Primenenie metoda PTsR v rezhime realnogo vremeni dlya vyyavleniya virusa afrikanskoy chumy sviney [Application of PCR in real time to detect African swine fever virus]. – Molekulyarnaya diagnostika. – Moscow, 2010. – pp. 83-85.

5. Gazayev I.Kh. Sovershenstvovanie metodov indikatsii genoma virusa afrikanskoy chumy sviney v obyektakh veterinarnogo nadzora [Improving methods of indication of genome of African swine fever virus in objects of veterinary surveillance]. – Pokrov, 2011. – p. 26.

6. Kopytov V.O., Tsybanova L.Ya., Selyaninov Yu.O., Tsybanov S.Zh. Identifikatsiya virusa afrikanskoy chumy sviney s pomoshchyu polimeraznoy tsepnoy reaktsii [Identification of African swine fever virus by polymerase chain reaction]. – Genodiagnostika infektsionnykh zabolevaniy. – Moscow, 2002. – pp. 341-343.

7. Kurinnov V.V. Razrabotka i usovershenstvovanie sredstv i metodov laboratornoy diagnostiki afrikanskoy i klassicheskoy chumy sviney [Development and improvement of means and methods of laboratory diagnosis of African and classical swine fever]. – Pokrov, 2000. – p.360.

8. Kurinnov V., Chernykh O., Mikolaychuk S. Vspyshka afrikanskoy chumy v khozyaystve zakryto gotipa (Krasnodarsky kray, noyabr 2008) [African swine fever outbreak in closed type farm (Krasnodar region, November 2008)]. – Zhivotnovodstvo Rossii. –Moscow, 2009 (4). – pp. 29-31.

9. Kurinnov V.V., Kolbasov D.V., Tsybanov S.Zh., Vasilev A.P. Afrikanskaya chuma sviney – glavnaya problema dlya svinovodstva Rossii [African swine fever – major problem for pig breeding of Russia]. – 2010: 82-87.

10. Kurinnov V.V., Kolbasov D.V., Tsybanov S.Zh. Diagnostika i monitoring pri vspyshkakh afrikanskoy chumy sviney v respublikakh Kavkaza v 2007-2008 [Diagnosis and monitoring of outbreaks of African swine fever in the Caucasian republics in 2007-2008]. – Veterinariya. – Moscow, 2008 (10). – pp. 20-25.

11. Tsybanov S.Zh., Vishnyakov I.F., Tsybanova L.Ya. Vyyavlenie virusa afrikanskoy chumy sviney s pomoshchyu polimeraznoy tsepnoy reaktsii (PTSR) [Identification of African swine fever virus by polymerase chain reaction (PCR)]. - Virusnye bolezni zhivotnykh. – Vladimir. – p. 26.

12. King D.P., Reid S.M., Hutchings G.H. Development of TaqMan PCR assay with internal amplification control for the detection of africam swine fever virus//J. Virol. Methods.-2003,vol.107,N°1, р.53-61.

13. S.Botija, and A.Ordas. Rapid diagnosis by identification of antibodies extracted from tissues using indirect immunofluorescence // Hog Cholera/Classical swine fever and African swine fever ,Luxembourg, 1977, c.658-659.

14. Steiger Y.,Ackermann M., Mettraux C., Kihm U. Rapid and biologically safe diagnosis of African swine fever virus infection by using polymerase chain reaction. J.Clin.Microbiol. 1992, (30), р.1-8.

15. Jose Manuel Sanchez-Vizcaino. African Swine Fever. В 9th Edition Diseases of Swine, Blackwell,2006,р.291-298.

Author affiliation

Kurinnov Viktor V., D.Sc. in Veterinary Medicine, professor, Diagnostics laboratory manager of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Vasilyev Aleksander P., Ph.D. in Biology, senior scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Belyanin S.A., Ph.D. in Veterinary Medicine, scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Strizhakova Olga M., Ph.D. in Veterinary Medicine, leading scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Nogina Irina V.,Ph.D. in Biology, chief scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Sidlik Marina V., Ph.D. in Biology, chief scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Gazaev Ismail Kh., Ph.D. in Biology, Genodiagnostics laboratory manager of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Tsybanov Sodnom Zh.,D.Sc. in Biology, Biophysics laboratory manager of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Mironova Lyudmila P., D.Sc. in Veterinary Medicine, professor, Director of the Rostov regional veterinary laboratory; 4, Akhtarsky lane, Rostov-on-Don, Rostov region, 344010; 8 (928) 1171431; e-mail: brucella@aaanet.ru.

Alikova Galina A., Ph.D. in Veterinary Medicine, Head of the Veterinary Committee of Volgograd region; 13, Gvardeysky st., Volgograd, Volgograd region, 400005; 8 (442) 30-98-04, 8(917)8493433; e-mail: vet@volganet.ru.

Dzhailidi Georgy A., Ph. D. in Biology, Head of the State Veterinary Department of Krasnodar region; 36, Rashpilevskaya st., Krasnodar, 350000; phone: 8 (861) 262-19-23; e-mail: dga@uv.krasnodar.ru.

Responsible for correspondence with the editorial board: Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin territorial veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; phone: 8(86138) 6-23-14; e-mail: gukkvl50@kubanvet.ru.

 

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