Lactic acid and sporing bacteria: formation of consortiums as the basis of functional supplements with probiotic properties for poultry farming

УДК 579.62
DOI 10.33861/2071-8020-2023-2-30-36

Mukhammadiev Rish.S., Mukhammadiev Rin.S., Valiullin L.R., Gibadullin R.Z., Gumerov V.G., Medetkhanov F.A.

Summary. The results of studies on the development of consortiums based on lactic acid and spore-forming bacteria, which open up prospects for their use as polyspecies additives with probiotic potential in poultry farming, are presented. We studied 6 bacterial strains that have antimicrobial properties against pathogens of intestinal infections and mycotoxicoses in young poultry. Their features of interaction were determined by surface (drop technique) and deep methods of cultivation. It was shown that the strains L. acidophilus IV138, P. acidilactici PA-12 and B. subtilis GA24 do not antagonize each other. Joint submerged cultivation of strains L. acidophilus IV138 and B. subtilis GA24 for 10 hours of growth led to an increase in the number of their cells, respectively, by 2.24 (4.27 x 109 CFU/ml) and 1.32 (1.00 x 109 CFU/ml) ml) times compared with the content of bacteria in monocultures (respectively 1.91 x 109 CFU/ml and 7.59 x 108 CFU/ml). After successive keeping of the selected strains in a medium with pH 3.0 for 1.5 hours (corresponding to the stomach of the bird) and 1% bile of broiler chickens for 6 hours (corresponding to the intestines), their survival rate was 29.6, 52.2 and 65.8 % relative to the number of microorganisms (per unit volume of suspension) at the beginning of the experiment. The selected bacterial strains had the ability to form various biologically active compounds. The bacillary strain was distinguished by a wide range of production of hydrolytic enzymes (cellulase, xylanase, amylase, pectinase, protease, and lipase) and antimicrobial metabolites (siderophores, bacteriocins, and exopolysaccharides). It should be noted that all analyzed bacteria formed compounds with antibacterial and antioxidant properties in the culture supernatant.

Keywords: Lactobacillus acidophilus, Pediococcus acidactici, Bacillus subtilis, strain, probiotic potential, bacterial communities, functional additives, poultry farming, broiler chickens.

References:

1. Molokanova O. V., Kurkin V. N., Hoshafyan L. S. et al. Alternativnoe reshenie po snizheniyu primeneniya v ptitsevodstve antibakterialnykh preparatov za schet korrektsii mikrobioty kishechnika ptitsy [Correction of intestinal microbiota as a mean to decrease the use of antibiotics in broiler production: practical experience]. - Pticevodstvo. - 2023 (3). - pp. 29-32.

2. Muhammadiev R. S., Muhammadiev R. S., Muhammadieva A. S. et al. Antagonisticheskaya aktivnost novykh shtammov mikroorganizmov v otnoshenii vozbuditeley kishechnykh infekciy molodnyaka selskohozyaystvennoy ptitsy [Antagonistic activity of new strains of microorganisms against intestinal infection pathogens of young farm poultry]. - Molodezhnye razrabotki i innovatsii v reshenii prioritetnykh zadach APK. - Kazan, 2022. - pp. 348-350.

3. Muhammadiev R. S., Muhammadiev R. S., Gumerov V. G. et al. Antagonisticheskiy potentsial molochnokislykh mikroorganizmov v otnoshenii vozbuditeley kishechnykh infekciy molodnyaka selskohozyaystvennoy ptitsy [Antagonistic potential of lactic acid bacteria against pathogens of intestinal infections in young poultry]. -Agropromyshlennyj kompleks: problemy i perspektivy razvitiya. -Blagoveshchensk, 2022. - pp. 138-144.

4. Muhammadiev R. S., Muhammadiev R. S., Gumerov V. G. et al. Antifungalnye svoystva novykh shtammov mikroorganizmov [Antifungal properties of new strains of microorganisms]. - Nauchnye razrabotki i innovatsii v reshenii strategicheskih zadach agropromyshlennogo kompleksa [Scientific developments and innovations in solving the strategic tasks of the agro-industrial complex]. - Izhevsk, 2022. -pp. 186-193.

5. Valiullin L. R., Mukhammadiev Rish. S., Mukhammadiev Rin. S. et al. Bakterii - antagonisty vozbuditeley kishechnykh infektsiy i produtsenty kompleksa tsellyulaz kak osnova dlya sozdaniya dobavok, obedinyayushchikh funktsii probiotika i kormovogo fermenta [Antagonistic bacteria of pathogens of intestinal infections and producers of a complex of cellulases as a basis for creating additives that combine the functions of a probiotic and a feed enzyme]. - Dostizheniya nauki i tekhniki APK. -2021 (35 (9). - рр. 60-66.

6. Mukhammadiev R. S., Mukhammadiev R. S., Karimullina I. G. et al. Biosovmestimost i fermentativnye svoystva novykh probioticheskikh shtammov mikroorganizmov [Biocompatibility and enzymatic properties of new probiotic strains of microorganisms]. - Sovremennye problemy eksperimentalnoy i klinicheskoy toksikologii, farmakologii i ekologii [Modern problems of experimental and clinical toxicology, pharmacology and ecology]. - Kazan, 2021. - рр. 232-236.

7. Khadieva G. F., Lutfullin M. T., Nikolaeva A. A. et al. Vliyanie probiotikov Bacillus subtilis GM2 i GM5 na rost i usvoyaemost kormov u tsyplyat-broylerov [Effect of Bacillus subtilis GM2 and GM5 probiotics on the growth and fodder digestibility of broiler chickens]. - Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki. - 2019 (161 (3). -рр. 472-489.

8. Mukhammadieva A. S., Mukhammadiev R. S., Mukhammadiev R. S. et al. Vydelenie i izuchenie morfologicheskikh i biohimicheskikh svoystv novykh shtammov molochnokislykh bakteriy, perspektivnykh dlya sozdaniya probioticheskikh preparatov [Isolation and investigation of morphological and biochemical properties of new strains of lactic acid bacteria promising for creation probiotic preparations]. - Veterinarnyy vrach. - 2020 (3). - рр. 39-46.

9. Osadchaya A. I., Safronova L. A., Poltavskiy A. N. et al. Gidrolaznaya aktivnost antarkticheskikh batsill [Hydrolase activity of antarctic bacilli]. -Mikrobiologiya i biotekhnologiya. - 2009 (4). - рр. 33-40.

10. Ushakova N.A., Kotenkova E.V., Kozlova A.A. et al. Izuchenie mekhanizmov probioticheskogo dejstviya shtamma Bacillus subtilis 8130 [A study of the mechanisms of probiotic effect of Bacillus subtilis strain 8130]. - Appl. Biochem. Microbiol. - 2006 (42(3)). - рр. 285-291. -DOI: 10.1134/S0003683806030057.

11. Khadieva G. F., Lutfullin M. T., Mochalova N. K. et al. Novye shtammy Bacillus subtilis kak perspektivnye probiotiki [New Bacillus subtilis strains as promising probiotics]. - Microbiology. - 2018 (87 (4). - рр. 463-471.

12. Valiullin L. R., Mukhammadiev Rish. S., Mukhammadiev Rin. S. et al. Novye shtammy Lactobacillus acidophilus kak perspektivnye probiotiki dlya pticevodstva [New Lactobacillus acidophilus strains as promising probiotics for poultry farming]. - Veterinariya Kubani. - 2022 (6). - рр. 16-21.

13. Mukhammadiev Rish. S, Valiullin L. R., Mukhammadiev Rin. S et al. Optimizatsiya sostava pitatelnoy sredy probioticheskogo shtamma B. subtilis GA24 - produtsenta kormovykh fermentov [Optimization of nutrient medium composition of probiotic strain B. subtilis GA24 - producer of forage enzymes]. - Uchenye zapiski Kazanskoy gosudarstvennoy akademii veterinarnoy mediciny im. N. E. Baumana. -2022 (250 (2). - рр. 155-159.

14. Tochilina A. G., Belova I. V., Ilicheva T. N. et al. Osobennosti genoma probioticheskikh bifidobakteriy, determiniruyushchie ikh shtammospetsificheskie svoystva [Genome features of probiotic Bifidobacteria determining their strain-specific properties]. - Sovremennye tekhnologii v meditsine. - 2022 (14 (5). - рр. 36-44.

15. Feoktistova N. V., Mardanova A. M., Khadieva G. F et al. Probiotiki na osnove bakteriy roda Bacillus v ptitsevodstve [Probiotics based on bacteria from the genus Bacillus in poultry breeding]. - Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki. - 2017 (159 (1). -рр.85-107.

16-25. Vide supra

Author affiliation:

Mukhammadiev Rinat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Sector of Probiotic Preparations and Enzymes of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-987-4214127; e-mail: tanirtashir@mail.ru.

Valiullin Lenar R., Ph.D. in Biology, senior scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; Head of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-950-9698469; e-mail: valiullin27@mail.ru.

Gibadullin Radik Z., Ph.D. in Biology, docent of the Department of Taxation and Economics of Forest Industry of the Kazan State Agrarian University; 65, Karla Marksa st., Kazan, 420015; phone: 8-917-2510182; e-mail: hflbru@list.ru.

Gumerov Vali G., D.Sc. in Veterinary Medicine, leading scientific researcher, Head of the Laboratory of Viral Animal Diseases of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-917-3976244; e-mail: gumerowali@mail.ru.

Medetkhanov Fazil A., D.Sc. in Biology, docent, Head of the Department of Pharmacology, Toxicology and Radiobiology of the Kazan State Academy of Veterinary Medicine named after N. E. Bauman; 35, Sibirskiy Trakt st., Kazan 420029; phone: 8-927-4178351; e-mail: ffazilak2@mail.ru.

Responsible for correspondence with the editorial board: Mukhammadiev Rishat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the AllRussian Scientific Research Institute of Phytopathology; scientific researcher of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-939-3728789; e-mail: tashir9891@mail.ru.