ÓÄÊ 579.62
DOI 10.33861/2071-8020-2023-2-30-36
Mukhammadiev Rish.S., Mukhammadiev Rin.S., Valiullin L.R., Gibadullin R.Z., Gumerov V.G., Medetkhanov F.A.
Summary. The results of studies on the development of consortiums based on lactic acid and spore-forming bacteria, which open up prospects for their use as polyspecies additives with probiotic potential in poultry farming, are presented. We studied 6 bacterial strains that have antimicrobial properties against pathogens of intestinal infections and mycotoxicoses in young poultry. Their features of interaction were determined by surface (drop technique) and deep methods of cultivation. It was shown that the strains L. acidophilus IV138, P. acidilactici PA-12 and B. subtilis GA24 do not antagonize each other. Joint submerged cultivation of strains L. acidophilus IV138 and B. subtilis GA24 for 10 hours of growth led to an increase in the number of their cells, respectively, by 2.24 (4.27 x 109 CFU/ml) and 1.32 (1.00 x 109 CFU/ml) ml) times compared with the content of bacteria in monocultures (respectively 1.91 x 109 CFU/ml and 7.59 x 108 CFU/ml). After successive keeping of the selected strains in a medium with pH 3.0 for 1.5 hours (corresponding to the stomach of the bird) and 1% bile of broiler chickens for 6 hours (corresponding to the intestines), their survival rate was 29.6, 52.2 and 65.8 % relative to the number of microorganisms (per unit volume of suspension) at the beginning of the experiment. The selected bacterial strains had the ability to form various biologically active compounds. The bacillary strain was distinguished by a wide range of production of hydrolytic enzymes (cellulase, xylanase, amylase, pectinase, protease, and lipase) and antimicrobial metabolites (siderophores, bacteriocins, and exopolysaccharides). It should be noted that all analyzed bacteria formed compounds with antibacterial and antioxidant properties in the culture supernatant.
Keywords: Lactobacillus acidophilus, Pediococcus acidactici, Bacillus subtilis, strain, probiotic potential, bacterial communities, functional additives, poultry farming, broiler chickens.
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Author affiliation:
Mukhammadiev Rinat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Sector of Probiotic Preparations and Enzymes of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-987-4214127; e-mail: tanirtashir@mail.ru.
Valiullin Lenar R., Ph.D. in Biology, senior scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; Head of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-950-9698469; e-mail: valiullin27@mail.ru.
Gibadullin Radik Z., Ph.D. in Biology, docent of the Department of Taxation and Economics of Forest Industry of the Kazan State Agrarian University; 65, Karla Marksa st., Kazan, 420015; phone: 8-917-2510182; e-mail: hflbru@list.ru.
Gumerov Vali G., D.Sc. in Veterinary Medicine, leading scientific researcher, Head of the Laboratory of Viral Animal Diseases of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-917-3976244; e-mail: gumerowali@mail.ru.
Medetkhanov Fazil A., D.Sc. in Biology, docent, Head of the Department of Pharmacology, Toxicology and Radiobiology of the Kazan State Academy of Veterinary Medicine named after N. E. Bauman; 35, Sibirskiy Trakt st., Kazan 420029; phone: 8-927-4178351; e-mail: ffazilak2@mail.ru.
Responsible for correspondence with the editorial board: Mukhammadiev Rishat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the AllRussian Scientific Research Institute of Phytopathology; scientific researcher of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-939-3728789; e-mail: tashir9891@mail.ru.
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