Indication of toxigenic Escherihia Coli using ciliates

UDC 579.842.11

Terekhov V.I.

Summary. Development of a method of biotesting of a toxigenicity of Escheriсhia coli with use of ciliates is the purpose of the study. E. coli strains isolated from animals with diarrhea symptoms and corpses were used. Existence of genes which are responsible for development of the thermolabile, thermostable, Shiga-like and necrotizing toxins is established using the method of polymerase chain reaction. Ciliates of 4 types − Paramecium caudatum, Stylonychia mytilus, Colpoda steinii and Tetrahymena pyriformis – are used as biological targets for these toxins. The environment of cultivation of escherichias – Hottinger’s broth, the environment of cultivation of infusorians − Lozina-Lozinsky. Results of research: it is established that with big degree of reliability specifically sensitive to toxins of escherichias were only Stylonychia mytilus infusorians. At addition in habitat of stilonikhiya of 24-hour cultures of the escherichias containing toxins there is a death from 12 to 45% of cages of infusorians. The most toxic effect E.coli − STX 1, 2 and SNF cytotoxins have, these products cause death of 23−45% of Stylonychia mytilus. To a lesser extent for infusorians of this look E.coli tsitotonina – TL and TS under the influence of which 12−22% of infusorians perish are. By a biotesting method on infusorians of Stylonychia mytilus it was determined that the maximum accumulation of cytotonics happens for 5−6 days, and cytotoxins – for 6−7 days.

Key words: Escherichia coli, toxigenicity, exotoxins, Shiga-toxin, thermo-labile toxin, thermostable toxin, biotesting, ciliates, Stylonychia mytilus.

References:

1. Vinokhodov D.O. Toksikologicheskie issledovaniya kormov s ispolzovaniyem infuzoriy [Toxicological studies of fodders using ciliates].–Saint-Petersburg, 1995. − pp.64-65.

2. EfanovaL.I., Manzhurina O.A., Stepanov A.V. Etiologicheskaya struktura faktornykh infektsiy sviney i krupnogo rogatogo skota v khozyaystvakh Rossii [Etiologic structure of factor infections of pigs and large horned cattle in farms of Russia].– Vestnik KSAA. – Kursk, 2012 (6). − pp.71−72.

3. Zaroza V.G. Esherikhioz telyat [Escherichiosis of calves]. –Agropromizdat. – Moscow, 1991. – 239 p.

4. Martynenko L.D.,Kalinina T.E., Lobanov V.V. Dinamika nakopleniya termolabilnogo enterotoksina E. coli O148 na zhidkikh pitatelnykh sredakh [Accumulation dynamics of heat labile enterotoxin E. coli O148 in the liquid nutrient media]. – Moscow, 1987. − pp. 89−92.

5. Novak D.D., Vulf V.T. Sposob polucheniya vaktsiny protiv esherikhioza telyat [Escherichiosis vaccine production method]. – Pat. 2070819. – Rossiyskaya Federatsiya, 27.12.1996.

6. Pautova I.G.,Vertiev Yu.V., Nikolayeva I.S. Izuchenie usloviy kultivirovaniya shtamma N19 E.coli dlya polucheniya shigellopodobnogo toksina [Study of E. coli strain H19 cultivation conditions for Shigella-like toxin]. – Moscow, 1987. – pp. 103-108.

7. Pirozhkov M.K. Biologicheskie preparaty dlya spetsificheskoy profilaktiki i terapii esherikhioza zhivotnykh [Biological preparations for specific prevention and treatment of animals at Escherichiosis]. –Moscow, 2002. –298 p.

8. Terekhov V.I.,Karayev Ya.M., Kogdenko N.V, Kotkova N.V. Antigenny sostav i patogennye svoystva shtammov E. coli, izolirovannykh ot telyat i porosyat v Krasnodarskom krae [Antigenic structure and properties of pathogenic strains of E. coli, isolated from calves and pigs in Krasnodar region]. – Rossiysky veterinarny zhurnal. – Moscow, 2008 (4). − pp. 6−8.

9. Shakhov A.E. Etiologiya faktornykh infektsiy zhivotnykh i mery ikh profilaktiki [Etiology of factor infections in animals and measures of prevention]. – Veterinarnaya patologiya. – 2005.− pp. 22−24.

10. El-Mahrouk I. Study of some virulence factors of E. coli from diarrheic sheep and goats / I.El-Mahrouk, A.M.O.M.G.Agour, A.M.Montasser // Bulletin of Animal Health and Production in Africa. −2012. −Т.60. −N°2. −P.153−163.

11. Rouillard T. Detection et identification par PCR des facteurs de virulence de souches d’Escherichia coli responsables de diarrhees neonatales / T. Rouillard, J.Guennec, P.Y. Moalic // Revista de Medicina Veterinaria. −2002. −Т.153. −N° 4. − P.261−268.

12. Truszczyński M. Vaccines against porcine colibacillosis, particularly after weaning / M. Truszczyński, Z. Pejsak //Medycyna Weterynaryjna. −2014. −R.70. − N°1. − S.3−6.

Author affiliation: Terekhov Vladimir I., D.Sc. in Bioljgy, professor, head of the department of the Krasnodar Station of fighting against animal diseases; 15/1, Kalinina st., Krasnodar, 350004; phone: 8(988)474-21-15; e-mail: vterekhov@list.ru – responsible for correspondence with the editorial board.