Estimation of Elisa diagnosis efficiency at swine trichinellosis

Verkhovsky O.A., Tsibezov V.V., Balandina M.V., Shevkoplyas V.N., Aliper T.I., Chernykh O.Yu.

Summary

Sensitive and specific serologic test kit based on solid-phase enzyme-linked immunosorbent assay (ELISA) named as "Trichinella-Serotest" has been evaluated for the detection of anti-Trichinella antibodies in porcine serum and meat juice samples. ELISA was developed using excretory-secretory antigens (E/S) from in vitro cultivated Trichinella spp. muscle larvae and the aim of this study was to compare ELISA results with taking results of artificial digestion method as gold standard. For this purpose 347 domestic pigs and 5 wild boars from different areas of Krasnodar region were examined for swine trichnellosis by both methods and positive results for 10 domestic pigs and 3 wild boars were obtained and analyzed. Results showed that all Trichinella-free domestic pigs were ELISA negative whereas sera from all Trichinella-infected animals contain specific antibodies which are detectable by ELISA. For wild boars it demonstrates that blood samples can be taken and tested simultaneously with meat juice samples by ELISA for confirmation diagnosis of trichinellosis. In conclusion, ELISA-kit "Trichinella-Serotest" is effective for veterinary laboratories and can be used for monitoring of swine Trichinella infection.

Key words: trichinellosis, domestic pigs, wild boars, enzyme-linked immunosorbent assay, ELISA-kit Trichinella-Serotest, sensitivity, specificity, diagnosis.

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Author affiliation

Tsibezov Valery V., Ph.D. in Biology, leading research associate of the Independent non-profit organization "Diagnostic and Prevention Research Institute for Human and Animal Diseases" (DPRI); 16, Gamaleya st., Moscow, 123098; phone: +7 (499) 190-75-61; e-mail: tsibezov@yandex.ru.

Balandina Marina V., Ph.D. in Biology, leading research associate of the Independent non-profit organization "Diagnostic and Prevention Research Institute for Human and Animal Diseases (DPRI); 16. Gamaleya st., Moscow, 123098; phone: +7 (499) 190-75-61; e-mail: info@dpri.ru.

Shevkoplyas Vladimir N., D.Sc. in Veterinary Medicine, professor of the department of veterinary-sanitary examination of the Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology; 23, Skryabina st., Moscow, 109472; phone: 8 (499) 784-62-19; e-mail: shevkoplyasvn@gmail.com.

Aliper Taras I., D.Sc. in Biology, professor, director of the NARVAC Ltd; 16, Gamaleya str., Moscow, 123098; phone: +7 (499) 190-30-54; e-mail: aliper@narvac.com.

Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin territorial veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; phone: +7 (86138) 6-23-14; e-mail: gukkvl50@kubanvet.ru.

Responsible for correspondence with the editorial board: Verkhovsky Oleg A., D.Sc. in Biology, professor, president of the Independent non-profit organization "Diagnostic and Prevention Research Institute for Human and Animal Diseases (DPRI)"; 16. Gamaleya st., Moscow, 123098; phone: +7 (499) 190-75-61; e-mail: info@dpri.ru.