Species composition of microorganisms isolated from tissues and organs of dead poultry

УДК 619:616.9:636.5
DOI 10.33861/2071-8020-2024-6-31-34

Mukhammadieva A. S., Mukhammadiev Rish. S., Karimullina I. G., Yarullin A. I., Muhammadiev Rin. S., Tarasova E. Yu., Khamidullina R. Z.

Summary. Failure to comply with the requirements necessary for raising poultry leads to the appearance of pathogenic and opportunistic microflora in their habitats, which, against the background of a decrease in the resistance of the animal’s body, can cause various infectious diseases or asymptomatic carriage. Considering the relevance of the problem, the purpose of this work was to isolate and identify microorganisms in pathological material from dead laying hens and broiler chickens kept in the private sector. The material for the pathological examination was the fresh corpses of dead 30 heads of broiler chickens and 20 heads of laying hens from 10 days to 2 months of age, delivered from different regions of the Republic of Tatarstan. Identification of isolated microorganisms was carried out using differential diagnostic nutrient media to study the nature of their growth, tinctorial and biochemical properties. According to morphological and cultural-biochemical indicators, the species composition of bacteria isolated from the corpses of dead broiler chickens included the pathogens E. coli, S. enteritidis, S. typhimurium, P. aeruginosa, P. mirabilis and S. aureus, from the corpses of dead chickens - E. coli, S. enteritidis, P. mirabilis and S. aureus. Enteropathogenic serogroups of E. coli had the highest occurrence, the predominant ones being O2 (73.4%), O78 (12.8%) and O115 (9.2%). Most of the isolated bacteria showed resistance to antimicrobial drugs such as benzylpenicillin, lincomycin, ampicillin with sulbactam, trimethoprim with sulfamethoxazole, vancomycin, cefuroxime, cefaclor, cefepime and ceftazidime. The bacteria were weakly sensitive to amoxicillin, fusidic acid, doxycycline, furadonin, cefotaxime and sensitive to ticarcillin with clavulanic acid, amoxicillin with clavulanic acid, gentamicin, ceftriaxone, and chloramphenicol.

Keywords: isolation, identification, microorganisms, species composition, dead birds, broiler chickens, laying hens, poultry farming.

Author affiliation:

Mukhammadiev Rishat S., Ph.D. in Biology, Senior Research Fellow, Laboratory of Feeds and Feed Additives, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-939-3728789; e-mail: tashir9891@mail.ru.

Karimullina Ilsiyar G., Ph.D. in Biology, Senior Research Fellow, Virology Laboratory, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-904-7699225; e-mail: 89047699225@mail.ru.

Yarullin Ainur I., Ph.D. in Biology, Leading Research Fellow, Department of Virological and Ultrastructural Research, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-905-3174170; e-mail: abii@mail.ru.

Mukhammadiev Rinat S., Ph.D. in Biology, Senior Research Fellow, Laboratory of Feeds and Feed Additives, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-987-4214127; e-mail: tanirtashir@mail.ru.

Tarasova Evgeniya Yuryevna, PhD in Biology, Leading Research Fellow, Veterinary Sanitation Laboratory, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-905-3779932; e-mail: Evgenechka1885@gmail.com.

Khamidullina Razina Zinnatullovna, Junior Researcher, Laboratory of Virology, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-952-0329511; e-mail: khamidullina140178@gmail.com.

Responsible for correspondence with the editorial board: Mukhammadieva Alina S., Ph.D. in Veterinary Medicine, Junior Researcher, Laboratory of Nutrient Media and Cell Cultures, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2, Kazan, 420075; phone: 8-917-2396095; е-mail: alinasakura_mail.ru@mail.ru.