Optimization of concentration conditions and purification of recombinant VP2 protein of infectious bursal disease virus

УДК 578.823:619
DOI 10.33861/2071-8020-2024-6-28-31

Veretennikov V. V., Tarlavin N. V., Kraskov D. A., Dzhavadov E. D., Rumyantsev A. M., Ishtuganova V. V.

Summary. The E. coli bacteria-based expression system is convenient and economical for the production of recombinant proteins on an industrial scale. In this work, a soluble VP2 protein of infectious bursal disease virus was produced. This protein can be used in the production of diagnostic tes-systems, but it is necessary to use purified recombinant protein to ensure high specificity of the assays performed, so in this study we described the technique of purification and concentration of the protein by its precipitation with saturated ammonium sulfate at different concentrations (20%, 40%, 60%). The recombinant VP2 protein was shown to be efficiently precipitated from cell lysate at an ammonium sulfate concentration of 40%. This contributed to the fact that the titer of recombinant protein in RDP did not decrease compared to the initial one, and the amount of impurity proteins in the solution decreased by about 50%.

Keywords: poultry farming, infectious bursal disease, recombinant VP2 protein, ammonium sulfate, diffusion precipitation reaction.

Author affliation:

Tarlavin Nikolay V., Ph. D. in Veterinary Medicine, Assistant of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8430353; e-mail: tarlav1995@bk.ru.

Kraskov Dmitry A., postgraduate student of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-921-4152266; e-mail: kraskov-00@bk.ru.

Dzhavadov Eduard D., D. Sc. in Veterinary Medicine, Professor of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-921-9666774; e-mail: vnivip1@mail.ru.

Rumyantsev Andrey M., Ph. D. in Biology, Scientific Researcher of the Department of Genetics and Biotechnology of the Saint-Petersburg State University; 7-9, Universitetskaya emb., Saint-Petersburg, 199034; phone: 8-911-2654652; e-mail: rumyantsev-am@mail.ru.

Ishtuganova Valeriya V., student of the Department of Genetics and Biotechnology of the Saint-Petersburg State University; 7-9, Universitetskaya emb., Saint-Petersburg, 199034; phone: 8-981-7473464; e-mail: st086860@student.spbu.ru.

Responsible for correspondence with the editorial board: Veretennikov Vladislav V., Ph. D. in Veterinary Medicine, Assistant of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8778769; e-mail: vlad.veretennikov.96@mail.ru.