Influence of AOKVET on milk bacterial contamination level in cows at mastitis

DOI 10.33861/2071-8020-2021-6-18-20

Kononenko K.N., Chekrysheva V.V., Klimenko A.I.

Summary. It is known that a determining role in the etiology of mastitis belongs to pathogenic and opportunistic microflora, which often include streptococci, staphylococci and much less often various enterobacteria. Pathogenic microorganisms are isolated more often in the form of associations, less often as monocultures out of milk samples from cows with signs of mammary gland lesions. Rational and effective approach to treatment of cows at mastitis primarily involves bacteriological studies including determination of the species composition of microflora and its sensitivity to antimastitis and individual antibacterial therapy. This work presents studies of 98 milk samples on the species composition of the microflora and its sensitivity to various drugs. Each sample was isolated on different types of media. Bacteriological examination of milk from cows with subclinical and clinical mastitis revealed 28 samples (27,4%) with staphylococci and streptococci; in 10 samples (10,2%) they were absent. Forty-four strains of microorganisms were isolated from the affected breast lobes, among which: 28 samples (13.7%) were classified as staphylococci; 16 samples (7.4%) as streptococci. In 53.1% of cases, streptococci and staphylococci were isolated simultaneously in mixed culture from the affected udder lobes. Staphylococci were isolated as monocultures from 12 milk samples (5.8%). Authors studied the dynamics of microbial contamination in milk before and after treatment of animals with AOKVET. The level of total microbial contamination of udder secretion decreased by 67% as a result of a single intracisternal AOKVET injection; after the third injection, the level of contamination in milk was minimal, which indicated the recovery of animals.

Keywords: cows, mastitis, milk contamination, AOKVET, antibacterial activity, pathogenic microorganisms, opportunistic microflora, streptococci, staphylococci, non-antibiotic agents.

References:

1. Batrakov A.Ya. Dinamika kontsentratsii antibiotikov v limfe vymeni korov pri arterialnom vvedenii ikh na fone novokainovoy blokady i bez nee [Dynamics of antibiotics' concentration in the lymph of the udder of cows with arterial injection of them against the background of novocaine blockade and without it]. - Leningrad, 1970. - pp. 126-128.
2. Zubenko A.A., Drobin Yu.D., Fetisov L.N., Bodryakov A.N. Sintez i skrining novykh soedineniy s vysokoy antimikrobnoy aktivnostyu [Synthesis and screening of new compounds with high antimicrobial activity]. - 2019: 207-215.
3. Rodin I.A., Kilmetova I.R., Sedov A.V., Kapustin A.V., Yakovets M.G. K voprosu o lechenii mastita u korov [On question of the treatment of mastitis in cows]. - 2019: 50.
4. Nastavlenie po diagnostike, terapii i profilaktike mastita u korov [Manual on the diagnosis, therapy and prevention of mastitis in cows]. - 2000: 11 p.
5. Drobin Yu.D., Kononenko K.N., Fetisov L.N., Zubenko A.A., Bodryakov A.N. Novyy preparat dlya lecheniya zhivotnykh pri mastitakh [New drug for treating animals with mastitis]. - Questions of legal regulation in veterinary medicine. - Saint-Petersburg, 2020 (1). - pp. 223-225.
6. Kononenko K.N., Kovalenko A.V., Fetisov L.N., Zubenko A.A., Bodryakov A.N. Preparat otechestvennogo proiskhozhdeniya dlya lecheniya mastitov [Preparation of domestic origin for mastitis treatment]. - Veterinaria i kormlenie. - Moscow, 2020 (7). - pp. 22-24.
7. Kovalenko A.V., Kononenko K.N., Fetisov L.N., Zubenko A.A., Klimenko A.I., Bodryakov A.N. Razrabotka novykh khimiopreparatov dlya lecheniya korov pri mastitakh [Development of new chemotherapy drugs for the treatment of cows with mastitis]. - Veterinaria i kormlenie. - Moscow, 2020 (6). - pp. 26-28.
8. Smirnova L.I., Sukhinin A.A., Belkina I.V., Prikhodko E.I., Feponova U.A. Sanitarno-mikrobiologicheskie issledovaniya moloka i molochnykh produktov [Sanitary and microbiological examination of milk and dairy products]. - Saint- Petersburg, 2009. - 54 p.
9. Drobin Yu.D., Zubenko A.A., Rodin I.A., Kononenko K.N., Fetisov L.N., Bodryakov A.N. Sposob lecheniya mastitov u zhivotnykh [Method for the treatment of mastitis in animals]. - RU 2711195 C1. - 2020.
10. Chekrysheva V.V. Diagnostika i ratsionalnye metody terapii pri klinicheskom mastite korov [Diagnostics and rational methods of therapy for clinical mastitis of cows]. - Novocherkassk, 2020.
11. 11-12. Vide supra.

Author affiliation:

Kononenko Kristina N., junior scientific researcher of the North Caucasian Zonal Scientific-Research Veterinary Institute - branch of the Federal Rostov Agrarian Scientific Center; 0, Rostovskoe hgw., Novocherkassk, Rostov region, 346421; phone: 8-918-5943886; e-mail: velikayakrista@mail.ru.

Klimenko Aleksandr I., D.Sc. in Agriculture, Academician of the Russian Academy of Sciences, professor, director of the Federal Rostov Agrarian Scientific Center; 1, Institutskaya st. Rassvet sttl., Aksayskiy district, Rostov region, 346735; phone: 8-86350-37389.

Responsible for correspondence with the editorial board: Chekrysheva Viktoria V., Ph.D. in Veterinary Medicine, director of the North Caucasian Zonal Scientific-Research Veterinary Institute - branch of the Federal Rostov Agrarian Scientific Center; 0, Rostovskoe hgw., Novocherkassk, Rostov region, 346421; phone: 8-908-5110139; e-mail: veterinar1987@mail.ru.