Antibiotic therapy effect on immuno-physiological status of fish

УДК 619:616.15:639.2.09:615.33
DOI 10.33861/2071-8020-2023-5-46-48

Romanova N. N., Sekhina O. V., Vishtorskaya A. A.

Summary. Changes in blood parameters after antibiotic therapy performed in juvenile carp and hybrid Siberian sturgeon х Kaluga (hereinafter, hybrid SS х K) are presented. A number of indices of the erythrocyte picture has been identified, which are non-specific markers, due to the fact that after a course of antibiotics in carp and hybrid SS х K, changes in the blood are different and even opposite, which indicates a species response. The leukocyte blood pattern shows similar changes in both carp and SS х K hybrid. The total number of leukocytes increases by almost 40%, which is a manifestation of stress leukocytosis associated with the release of glucocorticoids into the blood and the development of the second stage of the stress reaction - resistance (adaptation) in the body. Neutropenia and agranulocytosis were observed in fish treated with antibiotics, accompanied by an increase in the number of monocytes and lymphocytes. In addition, the hybrid SS х K increases the number of eosinophils, which can serve as a marker for sturgeon fish for a hypersensitivity reaction to antibiotics. To assess the reactivity of the body to antibiotic therapy, the leukocyte intoxication index was calculated, which decreases after a course of antibiotics in fish of experimental groups: in carp - by 2.75 times, in hybrid SS х K - by 1.95 times, relative to control groups. The greatest fluctuations in leukocyte intoxication index are manifested in carp, which corresponds to a more pronounced degree of intoxication of the body after antibiotic therapy. To correct the immuno-physiological status of fish after antibiotic therapy, it is advisable to conduct a feeding course with a probiotic drug.

Keywords: fish farming, antibiotic therapy, carp, sturgeon, hybrid of Siberian sturgeon х kaluga, immuno-physiological status, blood, hematology, blood parameters, leukocyte blood picture.

References:

1. Almazov V. et al. Fiziologiya leykotsitov cheloveka [Physiology of human leukocytes]. - Nauka, 1979. - 230 p.

2. Ivanova N. T. Atlas kletok krovi ryb [Fish Blood Cell Atlas]. - 1983: 184 p.

3. Batyrkhanova N. et al. Interpretatsiya laboratornykh dannykh (spravochnoe posobie) [Interpretation of laboratory data (reference book)]. - 2010: 40 p.

4. Metodicheskie ukazaniya po provedeniyu gematologicheskogo obsledovaniya ryb [Guidelines for conducting hematological examination of fish]. - 1999: 69-97.

5. Portnyagina E. V., Radnaev G. G. Ratsionalnaya antibakterialnaya terapiya i voprosy preduprezhdeniya bakterialnoy rezistentnosti: uchebnoe posobie [Rational antibacterial therapy and issues of preventing bacterial resistance: a textbook]. - IGMU, 2022: 135 p.

6. Romanova N. et al. Primenenie preparatov «Levofloksatsin» i «SUB-PRO» pri terapii bakterialnoy gemorragicheskoy septitsemii u karpovykh ryb [Use of Levofloxacine and SUB-PRO in treatment of bacterial hemorrhagic septicemia in cyprinid fish]. - Trudy VNIRO. - 2022 (190). - pp. 116-124.

7. Provotorov V., Ivanova G. Rol i mesto eritrotsitov v sisteme napravlennogo transporta razlichnykh farmakologicheskikh sredstv [Role and place of erythrocytes in the system of targeted transport of various pharmacological agents]. - Klinicheskaya meditsina, 2009. - №9. - S.4-8.

8. Bezyazychnaya A. A. et al. Osobennosti raspredeleniya tseftriaksona v organizme teplokrovnykh zhivotnykh [Features of the distribution of ceftriaxone in warm-blooded organism]. - Farmatsiya. - 2020 (6). - pp. 51-56.

9. Romanova N. et al. Otsenka vliyaniya probiotika SUB-PRO na imunno-fiziologicheskiy status ryb [Assessment of the effect of the probiotic SUB-PRO on the immune-physiological status of fish]. - 2020: 142-148.

10. Khem A., Kormak D. Gistologiya [Histology]. - Mir. - 1983. - 254 p. 11-14. Vide supra.

Author affiliation:

Romanova Natalia N., Ph. D. in Biology, docent, head of the laboratory of ichthyopathology of the branch for the freshwater fisheries of the All-Russian Research Institute of Fisheries and Oceanography; 40 a, Rybnoe sttl., Dmitrovsky urban district, Moscow region, 141821; phone: 8-495-1086856; e-mail: romanova_nn@vniiprh.ru.

Sekhina Olga V., specialist of the laboratory of ichthyopathology of the branch for the freshwater fisheries of the All-Russian Research Institute of Fisheries and Oceanography; 40 a, Rybnoe sttl., Dmitrovsky urban district, Moscow region, 141821; phone: 8-495-1086856; e-mail: sekhina_ov@vniiprh.ru.

Responsible for correspondence with the editorial board: Vishtorskaya Antonina A., specialist of the laboratory of ichthyopathology of the branch for the freshwater fisheries of the All-Russian Research Institute of Fisheries and Oceanography; 40 a, Rybnoe sttl., Dmitrovsky urban district, Moscow region, 141821; phone: 8-495-1086856; e-mail: vishtorskaya_aa@vniiprh.ru.