Application of the E-rosette method and detection of rosetto-forming lymphocytes in the blood and lymphoid organs of healthy and tb-sick cattle

УДК 619:616.98:579.873.21Т:636.2
DOI 10.33861/2071-8020-2023-5-7-9

Baratov M.O., Semenenko M.P.

Annotation. The aim of the research is a comparative study of the reactions of spontaneous rosette formation for the determination of T-lymphocytes in the blood of healthy and tuberculosis-infected cattle. We used lymphocytes isolated from the blood and lymphoid organs of 72 healthy cows and 96 tuberculosis cows. The most effective method was the isolation of lymphocytes on 17% verografin. To prepare the suspension, medium 199, Igla, on phosphate buffer and in Hank’s solution were used, of which Hank’s balanced solution (pH=7.2) and phosphate buffer (pH=7.2) turned out to be the most suitable. It was determined that the majority of tuberculous lymphocytes adsorbed 1620 ram erythrocytes (35-45% of the total number of rosette-forming lymphocytes) on their surface. The greatest number of these cells was noted in the thymus of animals with tuberculosis. The relative content of rosette-forming lymphocytes in the peripheral blood of healthy cows was 48.85±3.27%. In individual animals, the population of these cells ranged from 24% to 72%. A sharper decrease (2.33±1.20%) of rosette-forming lymphocytes was observed in the group with pathologically anatomically pronounced tuberculosis. The absolute number of rosettes increased depending on the degree of development of the tuberculous process. Thus, it was found that the blood lymphocytes of healthy cows form spontaneous rosettes. The number of T-lymphocytes in the blood and lymphoid organs of sick cows was significantly reduced. Further studies are needed to finally explain the obtained contradictory facts.

Keywords: T-lymphocytes, rosette-formation, tuberculosis, blast transformation, cattle, lymphoid organs, Ec-fragments of immunoglobulins, peripheral blood.

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Author affiliation:

Semenenko Marina P., D. Sc. in Veterinary Medicine, acting director of the Krasnodar Scientific Research Veterinaty Institute of the Krasnodar Research Centre for Animal Husbandry and Veterinary Medicine; 1, 1-ya Liniya st., Krasnodar, 350004; phone: 8-918-4612663; е-mail: sever291@mail.ru.

Responsible for correspondence with the editorial board: Baratov Magamed O., D. Sc. in Veterinary Medicine, chief scientific researcher, Head of the Laboratory of Infectious Pathology of the Caspian Zonal Research Veterinary Institute - a branch of the Federal Agrarian Research Center of the Republic of Dagestan, 367000, Makhachkala, st. Dakhadaeva, 88; phone: 8-928-5010948; е-mail: alama500@rambler.ru.