Obtaining recombinant capsid protein VP60 of rabbit haemorrhagic disease virus and its antigenic and immunogenic activity study

DOI 10.33861/2071-8020-2020-5-34-37

Mukhin A.N., Alekseev K.P., Moskvina A.S., Verkhovskiy O.A., Selezneva E.V., Chernykh O.Yu.

Summary. Viral hemorrhagic disease of rabbits is an acute, highly contagious disease characterized by the phenomena of hemorrhagic diathesis in all organs, especially in the lungs and liver. The causative agent of viral haemorrhagic disease of rabbits is a virus of haemorrhagic disease of rabbits, belonging to the family Caliciviridae, genus Lagovirus. Currently, there are 4 genogroups of lagoviruses, two pathogenic: GI1 (GI1a-GI1d) and GI2, and two non-pathogenic: GI3 and GI4. The greatest danger to rabbits in the Russian Federation is posed by viruses of the GI1 genotype. The virulence of these viruses for rabbits is extremely high, the incubation period is 48-72 hours. Clinically, the disease is almost not manifested. Mortality can reach 100%. For the prevention of HBV in the Russian Federation, inactivated tissue vaccines are used, which are a suspension of the liver of rabbits infected with virulent strains of the rabbit hemorrhagic disease virus. Currently, in veterinary practice, subunit recombinant vaccines based on virus proteins obtained in the baculovirus gene expression system are increasingly used. The authors obtained the recombinant VP60 virus of rabbit hemorrhagic disease of the GI1 genotype in the baculovirus gene expression system and studied its antigenic and immunogenic activity for rabbits. It was found that the recombinant capsid protein VP60 of the hemorrhagic disease virus, administered to rabbits at a dose of 50 pg, causes the synthesis of specific antibodies in animals, detected by enzyme immunoassay and in the hemagglutination inhibition reaction, and protects 80% of animals during control infection with the virulent strain «Voronezh-87» at a dose of 1000 LD 50. These data indicate the possibility of using this protein as a specific component of a subunit vaccine against rabbit hemorrhagic disease caused by strains of the GI1 gene group.

Keywords: rabbits, rabbit viral hemorrhagic disease, recombinant capsid protein, baculovirus gene expression system, antigenic activity, immunogenic activity, protection, specific antibodies, recombinant vaccine, virulent strain.

References:

1. Vlasova N.N, Vlasov N.A, Alekseev K.P. Konstruirovanie plazmidnogo vektora, ekspressiruyushchego VP60 virusa gemorragicheskoy bolezni krolikov [Construction of plasmid vector expressing VP60 of rabbit haemorrhagic disease virus]. - Veterinariya. - Moscow, 2006 (11). - pp. 53-55.

2. Raev S.A., Alekseev K.P., Shemelkov E.V., Musienko M.I., Orlyankin B.G., Mishin A.M., Verkhovskiy O.A., Zaberezhnyy A.D., Aliper T.I. Razrabotka i primenenie vaktsiny «VERRES-TSIRKO» [Development and application of the VERRES-CIRCO vaccine]. - Veterinariya segodnya. - Moscow, 2013 (3). - pp. 54-59.

3-12. Vide supra.

Author affiliation:

Alekseev Konstantin P., Ph.D. in Biology, Senior Scientific Researcher of the Scientific Research Institute for Diagnostics and Prevention of Human and Animal Diseases; 16, Gamaleya st., Moscow, 123098; phone: 8-495-7394216; e-mail: kalekseev@hotmail.com.

Moskvina Anna S., Ph.D. in Biology, Senior Scientific Researcher of the Scientific Research Institute for Diagnostics and Prevention of Human and Animal Diseases; 16, Gamaleya st., Moscow, 123098; phone: 8-495-7394216; e-mail: annamoskvina17@gmail.com.

Verkhovskiy Oleg A., D.Sc. in Biology, professor, president of the Scientific Research Institute for Diagnostics and Prevention of Human and Animal Diseases; 16, Gamaleya st., Moscow, 123098; phone: 8-495-7394216; e-mail: info@dpri.com.

Selezneva Ekaterina V., microbiologist of the Department of quality and control of Vetbiohim LLC; 16, Gamaleya st., Moscow, 123098; phone: 8-495-1935493; e-mail: selja07@rambler.ru.

Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin regional veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; тел.: 8-918-4956659; e-mail: gukkvl50@kubanvet.ru.

Responsible for correspondence with the editorial board: Mukhin Aleksey N., Ph.D. in Biology, Senior Scientific Researcher of the Scientific Research Institute for Diagnostics and Prevention of Human and Animal Diseases; 16, Gamaleya st., Moscow, 123098; phone: 8-495-7394216; e-mail: amuhin@yahoo.com.