Adaptation of sentinel method for biological control of African swine fever in pig farms

UDC 619:616.98:518.842.1

ADAPTATION OF SENTINEL METHOD FOR BIOLOGICAL CONTROL OF AFRICAN SWINE FEVER IN PIG FARMS

Dzhailidi G.A., Chernykh O.Yu., Kurinnov V.V., Strizhakova O.M.

Summary. Replication of the virus in the body of infected pigs at acute and subacute form of African swine fever is accompanied by excretion of the virus with nasal and lacrimal secrets, saliva, feces, urine already in the incubation period, and bleeding during the development of infection. The basic idea of biological control is the content of sentinel pigs in previously disadvantaged areas within 45 days, in the case of residual amounts of virulent ASF virus oral infection will happen through contact with contaminated surfaces, and it will be detected either by clinical signs (changes in behavior, loss of appetite and depression, increased body temperature above 40°C), or serologically by detection of specific antibodies, or laboratory methods at the end of the observation period. Piglets of rearing or first period of fattening group are to be bought only in the farm from one owner with confirmed health status in relation to acute viral (African swine fever, classical swine fever, Aujeszky’s disease), bacterial (erysipelas, pasteurellosis, hydropic disease) diseases, enterotoxaemia and poisoning. Sentinel piglets are labeled and placed regardless of the localization of the fixed outbreak and kept in compliance with all technological norms for at least 45 days (three 15-day incubation period). On the absence of ASF virus can be argued, if signs of disease or death were not detected after at least 45 days in pigs and results of laboratory methods with 100% sample of animals (absence of virus in the blood, confirmed with PCR and the absence of specific antibodies to ASF, vindicated indirect ELISA) were found to be negative. If biological control is carried out with negative results before mentioned 6 months, sentinel piglets are grouped and fed to marketable conditions, while sows, boars are moved to reproducer.

Keywords: African swine fever, pigs, virus virulence, incubation period, outbreak, virus inactivation, laboratory tests, antibodies, biological control, sentinel pigs method.

References:

1. Balyshev V.M., Kurinnov V.V., Tsybanov S.Zh., Kalantaenko Yu.F., Kolbasov D.V., Pronin V.V., Korneva G.V. Biologicheskie svoystva virusa afrikanskoy chumy sviney, vydelennogo v Rossiyskoy Federatsii [Biological properties of African swine fever virus isolated in the Russian Federation]. – Veterinariya. – Moscow, 2010 (7). – pp. 25–27.

2. Belyanin S.A., Vasilyev A.P., Kolbasov D.V., Tsybanov S.Zh., Balyshev V.M., Kurinnov V.V., Chernykh O.Yu. Virulentnost izolyatov virusa afrikanskoy chumy sviney [ASF virus isolates virulence]. – Veterinariya Kubani. – Krasnodar, 2011 (5). – pp. 9-10.

3. Belyanin S.A. Dinamika rasprostraneniya i monitoring epizooticheskogo protsessa afrikanskoy chumy sviney v Rossiyskoy Federatsii [Dynamics of distribution and monitoring of the epizootic process of African swine fever in the Russian Federation]. – Pokrov, 2013. – p.27.

4. Instruktsiya o meropriyatiyakh po preduprezhdeniyu i likvidatsii afrikanskoy chumy sviney [Instruction on measures to prevent and eliminate the African swine fever]. – 1980.

5. Kovalenko Ya.R. Afrikanskaya chuma sviney [African swine fever]. – Moscow, 1965. – 128 p.

6. Pravila provedeniya dezinfektsii i dezinvazii obyektov gosudarstvennogo veterinarnogo nadzora [Rules of disinfection and disinfestation of objects of state veterinary control]. – 2002.

7. Official Journal of the European Communities L 192/27 COUNCIL DIRECTIVE 2002/60/EC of 27 June 2002 laying down specific provisions for the control of African swine fever and amending Directive 92/ 119/EEC as regards Teschen disease and African swine fever.

8. OIE, Terrestrial Animal Health Code, 2014. –http://www.oie.int/.

Author affiliation:

Dzhailidi Georgy A., Ph. D. in Biology, Head of the State Veterinary Department of Krasnodar region; 36, Rashpilevskaya st., Krasnodar, 350000; phone: 8 (861) 262-19-23; e-mail: dga@uv.krasnodar.ru.

Kurinnov Viktor V., D.Sc. in Veterinary Medicine, professor, Diagnostics laboratory manager of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Strizhakova Olga M., Ph.D. in Veterinary Medicine, leading scientific researcher of the Diagnostics laboratory of the National Research Institute for Veterinary Virology and Microbiology; Pokrov, Petushki area, Vladimir region, 601120; (49243) 6-21-25; e-mail: vniivvim@niiv.petush.elcom.ru.

Responsible for correspondence with the editorial board: Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin territorial veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; phone: 8(86138) 6-23-14; e-mail: gukkvl50@kubanvet.ru.