Frequency of detection of bovine infectious rhinotracheitis virus ge-nome at pathology of reproduction in dairy farms

UDC 619:616.98:578.825.15:618.1/636.2 

Koteneva S.V., Semenova O.V., Glotova T.I., Glotov A.G., Koschaev A.G., Rodin I.A.

Summary. Data on study of serological status of animals to infectious bovine rhinotracheitis virus of cattle and frequency identifying viral genome by PCR-RFLP analysis were submitted. Widespread of IBR in large horned cattle in dairy farms of Siberia was found. Seropositivity of cows and heifers to IBR virus averaged 72.0% for farms: in small seropositivity was 64.2%, on average - 68%, in large - 86.1%. Genome of the virus was detected in samples of biological material from animals from farms unfavorable for gynecological pathology in 23.9% of those surveyed. Genome of the virus was present in the 26.9% of aborted fetuses of 6-8 months of age, 34% of stillborn calves, 22.4% of the samples of vaginal and uterine secretions, 36,4% of the samples of the placenta heifers and cows. In most cases the seroconversion to the virus after an abortion were detected in mothers. According to the results of PCR-RFLP analysis of 60% of the virus isolates classified as subtype 1.1. (Cooper-like), which corresponds to the restriction profile of «respiratory» strains; 33% - 1.2 subtype (TK-like), allocating at the genital form of the disease; 4% - to a group of «TK-A-like», with the nature of the vaccine attenuated strain; 3% of the isolates had a «mixed» restriction profile (Cooper- and TK-A-like), reflecting the co-circulation of the vaccine strains and field isolates of the virus. The presence of circulating virus in a susceptible population should be considered in the planning of control measures.

Keywords: infectious bovine rhinotracheitis, virus, large horned cattle, cows, pathology of reproduction, serological investigations, polymerase chain reaction, PCR-RFLP analysis.

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Author affiliation:

Koteneva Svetlana V., Ph.D. in Veterinary Medicine, senior scientific researcher of the Laboratory of Biotechnology of Diagnostic Center of the Siberian Federal Scientific Center of Agrobiotechnology; 463 PO box, Krasnoobsk vllg., Novosibirsk region, 630501; phone: 8-383-3087745; e-mail: koteneva-sv@mail.ru.

Semenova Olga V., Ph.D. in Biology, senior scientific researcher of the Laboratory of Virology of the Siberian Federal Scientific Center of Agrobiotechnology; 463 PO box, Krasnoobsk vllg., Novosibirsk region, 630501; phone: 8-383-3087745; e-mail: k-olga-83@mail.ru.

Glotova Tatyana I., D.Sc. in Biology, professor, Head of the Laboratory of Virology of the Siberian Federal Scientific Center of Agrobiotechnology; 463 PO box, Krasnoobsk vllg., Novosibirsk region, 630501; phone: 8-383-3087745; e-mail: t-glotova@mail.ru

Koshchaev Andrey G., D.Sc. in Biology, professor, Vice-Rector for Scientific Work of the Kuban State Agrarian University named after I.T Trubilin; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-2215637; e-mail: koshhaev.a@kubsau.ru.

Rodin Igor A., D.Sc. in Veterinary Medicine, professor of the department of anatomy, veterinary obstetrics and surgery of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8(861)2215835; e-mail: d22003807@mail.ru.

Responsible for correspondence with the editorial board: Glotov Aleksandr G., D.Sc. in Veterinary Medicine, professor, Head of the Laboratory of Biotechnology of Diagnostic Center of the Siberian Federal Scientific Center of Agrobiotechnology; 463 PO box, Krasnoobsk vllg., Novosibirsk region, 630501; phone: 8-383-3087745; e-mail: glotov_vet@mail.ru.