One-step protocol for thawing vitrified bovine blastocysts

УДК 619:636.2:611.013.31
DOI 10.33861/2071-8020-2024-4-23-25

Makutina V. A., Isaeva A. G., Krivonogova A. S., Moiseeva K. V., Romanova A. S.

Summary. The modified thawing (rehydration) protocol was first used for bovine blastocysts after vitrification. According to the results of blastocyst survival, as well as the rate of re-expansion after thawing, no significant differences were found between the modified and standard multi-stage protocol. Survival rates were similar in both groups (99.5%). The reexpansion rate was 2.22 ± 0.18 h in the control group and 2.34 ± 0.19 h in the modified protocol, respectively. The use of a modified protocol will reduce the time of embryo thawing, and therefore the time the embryos are outside the incubator at suboptimal temperature and osmolarity.

Keywords: vitrification, bovine blastocysts, thawing of embryos, thawing of blastocysts.

References:

1. Donnik I. M., Makutina V. A., Isaeva A. G. et al. Genetic technologies for preserving the bioresource potential of farm animals. - Veterinaria Kubani. - 2023 (4). - pp. 9-13.

2-9. Vide supra.

Author affiliation:

Makutina Valeriya A., Ph. D. in Biology, Senior Scientific Researcher of the Ural Federal Agrarian Research Center of the Ural Branch of the RAS; 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-343-2572044; e-mail: makutina_v@rambler.ru.

Krivonogova Anna S., D. Sc. in Biology, Docent, Leading Scientific Researcher of the Ural Federal Agrarian Research Center of the Ural Branch of the RAS; 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-343-2572044; e-mail: tel-89826512934@yandex.ru.

Moiseeva Kseniya V., Junior Scientific Researcher of the Ural Federal Agrarian Research Center of the Ural Branch of the RAS; 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-343-2572044; e-mail: moiseeva456@yandex.ru.

Romanova Alisa S., Ph. D. in Technics, Senior Scientific Researcher of the Ural Federal Agrarian Research Center of the Ural Branch of the RAS; 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-343-2572044; e-mail: alisic_kolotova@mail.ru.

Responsible for correspondence with the editorial board: Isaeva Albina G., D. Sc. in Biology, Docent, Leading Scientific Researcher of the Ural Federal Agrarian Research Center of the Ural Branch of the RAS; 112 a, Belinskogo st., Ekaterinburg, 620142; phone: 8-343-2572044; e-mail: isaeva.05@bk.ru.