Assessment of bacterial inhibition action of colloidal solutions of silver and silicium nanoparticles by diffusion method

DOI 1033861/2071-8020-2019-4-15-17

Krasochko P.A., Korochkin R.B., Krasochko P.P., Ponaskov M.A., Koshchaev A.G., Chernykh O.Yu.

Summary. Currently, nanotechnology and nanomaterials have found application in many areas of human life and activity, and nanoscale silver particles are of particular interest due to their antibacterial activity. Currently, there are many manufacturers of preparations based on nanoparticles with bioelement, but the routine methods of laboratory assessment of their antibacterial action differ in their execution. Nanoparticles are considered the most effective alternative or addition to antibiotics, having high potential for solving the problem of the emergence of bacteria with multiple antibacterial resistance. Authors studied the action of bioelement nanoparticles produced by CJSC Concern Nanoindustry of the Institute of Nanotechnology IFC (Russian Federation) samples of AgBion-2 and silicon dioxide preparations. Inhibitory effect of these drugs on the growth of microorganisms of various species was evaluated: Klebsiella pneumonia ATCC 700603, Escherichia coli ATCC 25922, Salmonella enterica subsp. enterica ATCC BAA-2162, Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027. Authors showed that due to the lack of a standardized method for assessing the antibacterial effect of nanoparticle solutions by the size of the zone of growth inhibition of microorganisms, the experiments obtained in the inhibition zone do not allow categorizing the degree of sensitivity of test microorganisms to the tested nanoparticle solutions. Authors showed that of all the tested microorganisms, the highest sensitivity was demonstrated by Staphylococcus aureus ATCC 6538, in relation to which the values of ring radii of growth inhibition were more than 15.5 mm. Salmonella enterica subsp. had an annular radius of growth retardation of 8.0 mm in working dilution, and the sensitivity of the remaining microorganisms was approximately the same (within 3.0-3.5 mm). This result contradicts previous studies, where it was shown that the sensitivity of gram-negative microorganisms to nanoparticles of bioelements is higher than that of gram-positive.

Keywords: nanoparticles, silver, silicon dioxide, antibacterial activity, growth inhibition, test microorganisms, antitoxic effect, categorization, degree of sensitivity.

References:

1. Krasochko P.A. et al. Sposob opredeleniya antagonisticheskoy aktivnosti antibakterialnogo preparata. - Patent RB 19949. - Minsk, 2015. - 4 p.

2. Krasochko P.A. et al. Sposob opredeleniya antagonisticheskoy aktivnosti antibakterialnogo beskletochnogo probioticheskogo preparata. - Patent RB 19955. - Minsk, 2015. - 4 p.

3-10. Vide supra.

Author affiliation:

Krasochko Petr A., D.Sc. in Veterinary Medicine, D.Sc. in Biology, Professor, Academician of the Academy of Natural Sciences, Head of the Department of Epizootology and Infectious diseases of the Vitebsk State Academy of Veterinary Medicine; 7/11, Dovatora st., Vitebsk, Republic of Belarus, 210027.

Korochkin Rudolf B., Ph.D. in Veterinary Medicine, Docent of the Department of Microbiology and Virology of the Vitebsk State Academy of Veterinary Medicine; Vitebsk, Republic of Belarus; 7/11, Dovatora st., Vitebsk, Republic of Belarus, 210027; e-mail: rudolfvit@mail.ru.

Krasochko Pavel P., Ph.D. in Veterinary Medicine, Docent, Senior Scientific Researcher of the Vitebsk State Academy of Veterinary Medicine; Vitebsk, Republic of Belarus; 7/11, Dovatora st., Vitebsk, Republic of Belarus, 210027.

Ponaskov Mikhail A., Master in Veterinary Medicine, post-graduate student of the Department of Epizootology and Infectious diseases of the Vitebsk State Academy of Veterinary Medicine; 7/11, Dovatora st., Vitebsk, Republic of Belarus, 210027.

Koshchaev Andrey G., D.Sc. in Biology, Professor, Vicerector of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-2215637; e-mail: koshhaev.a@kubsau.ru.

Responsible for correspondence with the editorial board: Chernykh Oleg Yu., D.Sc. in Veterinary Medicine, director of the Kropotkin regional veterinary laboratory; 303, Krasnoarmeyskaya st., Kropotkin, 352380; phone: 8-918-4956659; e-mail: gukkvl50@kubanvet.ru.