Syncytial method at early identification of virus of bovine leucose

UDC 619:616-07:578. 828.2/001.8:636.2

Donnik I.M., Petropavlovskii M.V.

Summary. Positive results are obtained on the study of the diagnostic value of syncytial test for early detection of virus carriers of bovine leukemia for the first time in the Urals region. Intraperitoneal infection of laboratory animals (sheep and goats) with leucocyte suspension containing viral material was carried by the authors. Group of isolated contained animals was formed for comparative diagnostic study; it was weekly investigated by serological, molecular-genetic, cultural diagnostic studies, beginning from the sixth day after infection. Laboratory studies confirmed the diagnostic value of direct methods for the identification of the causative agent of leucose of large horned cattle. Positive reaction in syncytial method of research, which was confirmed by controls, was detected in the earliest period (13 days) after artificial infection of laboratory animals. Positive PCR was revealed in 21 days, while reaction of immune diffusion and ELISA – only on the 37th day (5th week). Early developed diagnosis of leucose will increase the efficiency of improvement of the agricultural enterprises of the infection, as the isolation of infected animals at the earliest timing is one of the priorities in the implementation of health programs.

Key words: leucose of large horned cattle, retrovirus, diagnostics, syncytial test, RID (reaction of immune diffusion), ELISA, PCR (polymerase chain reaction), cell culture, early identification of the virus.

References:

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Author affiliation:

Donnik Irina M., D.Sc. in Biology, Academician of RAAS, professor, rector of the Urals State Agrarian University; 42, Karla Libknekta st., Yekaterinburg, Sverdlovsk area, 620075; phone: 8 (343) 371-33-63; e-mail: rector@urgau.ru.

Responsible for correspondence with the editorial board: Petropavlovskii Maksim V., Ph.D. in Veterinary Medicine, Senior Scientific Researcher of the Ural Scientific Research Veterinary Institute; 112a, Belinskogo st., Yekaterinburg, Sverdlovsk region, 620219; phone: 8 (343) 2572044; e-mail: info@urnivi.ru.