Optimization of co-cultivation parameters of Bacillus Subtilis GA27 and Bacillus Subtilis RF-45 for possibility of creating probiotics, metaprobiotics, and metabiots for poultry farming

УДК 579.62
DOI 10.33861/2071-8020-2023-3-32-38

Mukhammadiev Rish.S., Mukhammadiev Rin.S., Valiullin L.R., Baryshev M.G., Yarullin A.I., Dupleva L.Sh., Karimullina I.G., Timerbaeva R.R.

Summary. The results of studies on optimizing the parameters of co-cultivation of B. subtilis GA27 and B. subtilis RF-45 strains are presented in the article. The greatest accumulation of the biomass of the studied bacillary strains, corresponding to their maximum antagonistic action against pathogens of intestinal infections in young poultry, was provided on a soy medium with the addition of 0.5% wheat bran at a temperature of 35°C, the active acidity of the medium was 6.5, the rotation speed of the rocking chair was 180 rpm, the duration of the process of their joint deep cultivation is 16 hours. In this case, stationary cultures with a total titer of at least (6.12 ± 0.17) х 106 CFU/ml should be used as seed material and applied in an amount of 10% on the volume of the medium for growth. The optimal ratio of the seed concentration of B. subtilis GA27 and B. subtilis RF-45 was 1:2. The cell suspension, culture liquid, and supernatant obtained after co-cultivation of bacilli were characterized by the ability to correct the intestinal microbiota of Wistar rats with gentamicin-induced dysbiosis. Compared with the cell suspension and the culture supernatant of the bacterial association, the culture liquid of the latter had the highest recovery rate of the intestinal microbiota of animals. Under the influence of the cultural liquid of the bacillary consortium, the rate of recovery of the total number of intestinal microorganisms relative to the similar indicator of the experimental groups of animals that received its cultural supernatant and cell suspension was 1.12 and 8.35 times higher, respectively, the number of lactic acid bacteria - 1.04 and 13.03 times, respectively. The cell suspension, culture liquid and supernatant of the association B. subtilis GA27 and B. subtilis RF-45 exceeded the indicated indicator of the control group of animals by 22-245 times in terms of the recovery rate of bacteria of the E. coli group, the most effective in this respect was the culture supernatant of the bacterial consortium.

Keywords: Bacillus subtilis, biocompatible strains, co-cultivation, optimization, antimicrobial potential, correction of intestinal microecological disorders, efficiency, probiotics, metaprobiotics, metabiotics, poultry farming.

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Author affiliation:

Mukhammadiev Rinat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Sector of Probiotic Preparations and Enzymes of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-987-4214127; e-mail: tanirtashir@mail.ru.

Valiullin Lenar R., Ph.D. in Biology, senior scientific researcher of the Department of Ecology of the All-Russian Scientific Research Institute of Phytopathology; Head of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-950-9698469; e-mail: valiullin27@mail.ru.

Baryshev Mikhail G., D.Sc. in Biology, Professor, Professor of the Russian Academy of Sciences, Acting Director of the All-Russian Scientific Research Institute of Phytopathology; 5, Institutskaya st., Bolshie Vyazemy, Odintsovo district, Moscow region, 143050; phone: 8-495-5974228; e-mail: vniif@vniif.ru.

Yarullin Ainur I., Ph.D. in Biology, Senior Research Fellow, Department of Virological and Ultrastructural Research, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-905-3174170; e-mail: abii@mail.ru.

Dupleva Liliya Sh., Ph.D. in Biology, Senior Research Fellow, Department of Bacteriology, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-917-2782737; e-mail: dupleva.lilya@mail.ru.

Karimullina Ilsiyar G., Ph.D. in Biology, Senior Research Fellow, Department of Virological and Ultrastructural Research, Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-904-7699225; e-mail: 89047699225@mail.ru.

Timerbayeva Razaliya R., Ph.D. in Veterinary Medicine, Associate Professor of the Department of Epizootology and Parasitology, Kazan State Academy of Veterinary Medicine named after N.E. Bauman; 35, Sibirskiy Trakt st., Kazan, 420029; phone: 8-903-3401420; e-mail: 8-903-3401420@mail.ru.

Responsible for correspondence with the editorial board: Mukhammadiev Rishat S., Ph.D. in Biology, scientific researcher of the Laboratory of Applied and Experimental Microbiology of the All-Russian Scientific Research Institute of Phytopathology; scientific researcher of the Laboratory of Feeds and Feed Additives of the Federal Center for Toxicological, Radiation and Biological Safety; Nauchnyy gorodok-2 st., Kazan, 420075; phone: 8-939-3728789; e-mail: tashir9891@mail.ru.