Study of the effect of various vaccination regimens of chickens against H5N1 subtype highly pathogenic influenza on metabolic and humoral immunity parameters

УДК 577.112:612.1:636.5-053.2:616.98:578.832.1-085.371
DOI 10.33861/2071-8020-2025-2-6-9

Original Empirical Research

Vasilyeva S.V., Tarlavin N.V., Veretennikov V.V., Khripunkova D.S.

Abstract. The article describes the effects of different schemes of application of the experimental candidate vaccine from the recombinant reassortant strain A/Syktyvkar/PR8/6:2/HA20 (H5N1) on the immuno-biochemical processes of chickens. Male chickens of the Dekalb White egg cross were vaccinated once and twice with an interval of 14 days, starting from the first or tenth day of life. In immunized chickens at the age of 45 days, there were changes of varying degrees in the most important metabolic parameters, which were manifested in an increase in the intensity of catabolic processes. The most pronounced unfavorable changes - an increase in the concentration of urea, uric acid against the background of a decrease in the level of cholesterol and albumin - were found in chickens that began to be vaccinated on the tenth day of life. At the same time, in chickens immunized twice, these changes were more noticeable and were statistically significant in comparison with the control group. Correlation analysis performed by comparing the average group metabolic indices revealed a strong negative relationship between cholesterol and nitrogen metabolism products - urea and uric acid, which confirms the dependence of anabolic and catabolic processes in growing chickens on the immunization scheme used. Vaccination started at the age of one day had a lesser effect on systemic metabolism, while a single administration of the vaccine had almost no effect on the protein, carbohydrate and lipid metabolism of the bird. All the vaccination schemes used caused an increase in the concentration of total immunoglobulins by 65.9-81.6% and an increase in the extinction of circulating immune complexes by 3.3-8.7 times. The data obtained provided important information on the reaction of the chickens’ organism to various vaccination schemes against the H5N1 influenza virus for one and a half months, which will help in the future to find optimal conditions for the formation of intense immunity.

Keywords: poultry farming, epizootology, highly pathogenic avian influenza, metabolic processes, biochemistry of poultry blood, vaccination.

Author affiliation:

Vasilyeva Svetlana V., Ph.D. in Veterinary Medicine, Docent of the Department of Biochemistry and Physiology of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-904-6399033; e-mail: svvet@mail.ru.

Veretennikov Vladislav V., Ph.D. in Veterinary Medicine, Senior Lecturer of the Department of Epizootology named after V.P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8778769; e-mail: vlad.veretennikov.96@mail.ru.

Khripunkova Darya S., 4th year student of the Faculty of Veterinary Medicine of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-904-6953600; e-mail: khripunkova.d1612@gmail.com.

Responsible for correspondence with the editorial board:Tarlavin Nikolay V., Ph.D. in Veterinary Medicine, Senior Lecturer of the Department of Epizootology named after V.P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8430353; e-mail: tarlav1995@bk.ru.

Authors’ Contribution:

Vasilyeva S.V.: conceptualization, data curation, methodology, supervision, review and editing.

Veretennikov V.V.: formal analysis, validation.

Tarlavin N.V.: funding acquisition, project administration, resources.

Khripunkova D.S.: investigation, visualization, original draft preparation.

Conflict of Interest Statement: the authors declare no conflict of interest.