Influence of the bacteriophage preparation on the opportunistic pathogenic microflora of broilers

УДК 619:636.5.033:578.347
DOI 10.33861/2071-8020-2023-2-37-40

Krivonogova A. S., Isaeva A. G., Donnik I. M., Loginov E. A., Musikhina N. B.

Summary. Authors studied the possibility of using bacteriophages to correct the composition of the microbiocenosis of the gastrointestinal tract in broiler chickens. The experimental group of chickens was fed a preparation containing bacteriophages active against opportunistic and enteropathogenic bacteria - S. flexneri, S. sonnei, S. typhimurium, S. flexneri, Salmonella, E. coli, Proteus, Enterococcus, Staphylococcus and P. aeruginosa. It was found that the use of bacteriophage preparations had a positive effect on the composition of the intestinal microflora of broilers, reducing the proportion of clinically significant opportunistic bacteria in its structure, including those with antibiotic resistance. After the use of bacteriophages, the frequency of occurrence of isolates of Staphylococcus spp., Enterococcus faecium, Escherichia fergusonii, Klebsiella spp. in the biomaterial of chickens of the experimental group. After application of bacteriophages in the experimental group the average susceptibility of Staphylococcus spp. isolates to studied antibiotics increased: to ciprofloxacin by 2.5 times, to doxycycline - 2 times, to tobramycin - 2.8 times, to enrofloxacin - 2.6 times. In the control group, a decrease in the susceptibility of staphylococci to ciprofloxacin and doxycycline was noted. The number of MDR isolates of Staphylococcus and E. coli decreased in the experimental group and increased in the control group. At the same time, in broilers treated with the bacteriophage, a positive trend was noted in the increase in live weight by the time of slaughter. At the same time, the average value of live weight was higher by 149 g in the experimental group that received the bacteriophage. Thus, the use of bacteriophages in productive poultry of meat crosses can be promising for correcting microbiocenoses and reducing their contamination with antibioticresistant isolates, as well as for preventing dysbiosis.

Keywords: opportunistic bacteria, bacteriophage, broiler chickens, antimicrobial resistance, intestinal microflora, microbial contamination.

References:

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Author affiliation:

Krivonogova Anna S., D.Sc. in Biology, docent, Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: tel-89826512934@yandex.ru.

Donnik Irina M., D.Sc. in Biology, professor, Academician of the Russian Academy of Sciences, head of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: ktqrjp7@yandex.ru.

Loginov Egor A., postgraduate student of the Faculty of Veterinary Medicine of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: loginov.ea19@gmail.com.

Musikhina Nina B., senior lecturer of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; е-mail: musikhina83@mail.ru.

Responsible for correspondence with the editorial board: Isaeva Albina G., D.Sc. in Biology, docent, professor of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: isaeva.05@bk.ru.