Method for detecting shiga toxin-producing E. Coli bacteria in animal feed

УДК 579.62
DOI 10.3861/2071-8020-2025-1-8-10

Original Empirical Research

Popov P.A., Bannikova L.A., Babunova V.S., Tyumentseva V.S.

Abstract. Shiga toxin derived E. coli pose a significant danger to human health. The clinical manifestations of EHEC infections range from asymptomatic carriage to hemorrhagic colitis and hemolytic-uremic syndrome. More severe consequences are caused by enterohemorrhagic E. coli, which includes the O157:H7 serogroup. Classical strains of the ENES group contain EAE gene, which encodes the synthesis of the protein of the outer membrane of the intimin cell, involved in the adhesion of the pathogen to the surface of the intestinal epithelium, as well as the HLY gene, which controls the production of enterohemolysin. Acute intestinal infections caused by Shiga toxin-producing E. coli of various serogroups, including E. coli O157:H7, are registered almost everywhere. Shiga toxins are encoded and distributed by converting lambdoid bacteriophages called Stx phages. In addition to increasing the pathogenic profiles of virulent E. coli strains, they can also turn commensal members of the Enterobacteriaceae family in the intestine into producers of a huge variety of toxins due to horizontal transfer and incorporation into the bacterial genome.

Keywords: Escherichia coli bacteria, Shiga toxin, feed, detection method, verification.

Author affiliation:

Bannikova Daria A., Ph. D. in Veterinary Medicine, Leading Scientific Researcher of the Laboratory of Sanitary Microbiology of the All-Russian Scientific Research Institute of Veterinary Sanitation, Hygiene and Ecology – branch of the Federal Research Center for Experimental Veterinary Science of the Russian Academy of Sciences; 5, Zvenigorodskoe hgw., Moscow, 123022; e-mail: andreevna.07@mail.ru.

Babunova Veronika S., Ph. D. in Veterinary Medicine, Leading Scientific Researcher of the All-Russian Scientific Research Institute of Veterinary Sanitation, Hygiene and Ecology – branch of the Federal Research Center for Experimental Veterinary Science of the Russian Academy of Sciences; 5, Zvenigorodskoe hgw., Moscow, 123022; e-mail: veronikavniivs@mail.ru.

Tyumentseva Valeriya S., Scientific Researcher, postgraduate student of the All- Russian Scientific Research Institute of Veterinary Sanitation, Hygiene and Ecology – branch of the Federal Research Center for Experimental Veterinary Science of the Russian Academy of Sciences; 5, Zvenigorodskoe hgw., Moscow, 123022; e-mail: 89296712838v@gmail.com.

Responsible for correspondence with the editorial board: Popov Petr A., D. Sc. in Veterinary Medicine, Head of the All-Russian Scientific Research Institute of Veterinary Sanitation, Hygiene and Ecology – branch of the Federal Research Center for Experimental Veterinary Science of the Russian Academy of Sciences; 5, Zvenigorodskoe hgw., Moscow, 123022; e-mail: popov.petr18@gmail.com.

Conflict of Interest Statement: the authors declare no conflict of interest.