Dynamics of antibiotic susceptibility of Enterococcus faecium strains at the dairy farms in the regions with various levels of agrocoenosis contamination

DOI 10.33861/2071-8020-2019-1-7-10

Donnik I.M., Isaeva A.G., Bykova O.A., Lysova Ya.Yu., Moiseeva K.V., Krivonogova A.S.

Summary. Results of the research on opportunistic pathogenic microflora at the dairy farms located in three regions having various levels of contamination with industrial pollutants are described in the article. The research was done on the structure of opportunistic pathogenic part of microbiocenosis at the farms and susceptibility of strains of the most common microorganisms to antibiotics. The detailed analysis of antibiotic susceptibility of Enterococcus faecium taken from various objects at the dairy farms in the control and experimental areas. The data on the status of antibiotic susceptibility of enterococcus for the year of 2018 have been updated. Interconnection between the level of technogenic environmental contamination and quantity of enterococcus strains with low susceptibility and resistance to one, two, three and more antibiotics of various types was stated. The research was done on the tendency in expansion of multiresistant Ent. faecium strains at livestock enterprises. The characteristics of susceptibility of enterococcus strains to vancomycin was given. In the course of the research it was stated that the profile of antibiotic susceptibility, common for VRE-strains, was only found in enterococcus from the man-made contaminated regions. In the samples from the farms located in the control region with the minimal level of man-made pollution, no facts of resistance of enterococcus to vancomycin were observed. Results of the research done have shown that formation and expansion of resistant strains of enterococcus was more intensive at the farms located in environmentally neglected zones with significant contamination of air, soil, water and forage plants with toxic metals and human-made radionuclides. The research was supported by a grant from the Russian Science Foundation (Project No. 18-16-00040).

Key words: antibiotic susceptibility, antibiotic resistance, opportunistic pathogenic microflora, Enterococcus faecium, Enterococcus faecalis, vancomycin-resistant enterococcus, cattle, anthropogenic pollution, heavy metals, radionuclides.

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Author affiliation:

Donnik Irina M., Vice-president of the Russian Academy of Sciences, head of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: ktqrjp7@yandex.ru.

Bykova Olga A., D.Sc. in Agriculture, docent, head of the office for scientific research of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-2956131; e-mail: olbyk75@mail.ru.

Lysova Yana Yu., scientific researcher of the Institute of Biosafety of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-922-1532003; e-mail: boris.wet@mail.ru.

Moiseeva Ksenia V., assistant of the Department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-953-0475887; e-mail: moiseeva456@yandex.ru.

Responsible for correspondence with the editorial board: Isaeva Albina G., D.Sc. in Biology, docent, professor of the department of infectious and non-infectious pathology of the Ural State Agrarian University; 42, K. Liebknekhta st., Yekaterinburg, 620075; phone: 8-343-371-33-63; e-mail: isaeva.05@bk.ru.