Nechaeva O.V., Tikhomirova E.I., Laskavyy V.N., Kuznetsova A.E., Vakaraeva M.M.
Summary. The search for new drugs for the treatment of infectious diseases of viral etiology is one of the urgent tasks of modern veterinary medicine. In addition to high efficiency, antiviral drugs should be safe to use and not have a negative impact on the macroorganism. The article presents the results of an investigation of the antiviral activity of a heterocyclic compound of a series of phenylpentendione-2,4-dichloro-1,3,5-triphenyl-2-pentene-1,5-dione and a biocompatible polymer compound, polyazolidine ammonium, modified with hydrated iodine ions. The choice of these compounds was due to their low level of toxicity and a broad spectrum of antimicrobial activity. As an experimental model, the strain «BH-96» of porcine porcine gastroenteritis virus was used, which was cultivated on the transplantable kidney cell line of a pig embryo. Using the Reed and Mench method, sub-toxic doses of the test compounds were determined, which were 1 Mg / ml. Experimental samples of the cell culture were pretreated with working concentrations of the test compounds followed by the formation of a monolayer. In the course of the conducted studies, it was revealed that in the control samples of the monolayer of cell culture infected with the virus, significant destructive changes of cells occurred on the third day of cultivation, with their detachment from the substrate to the fifth day, when the cell damage of the virus was 80-90%. Preliminary treatment of the cell culture with subtitic concentrations of the test compounds led to the preservation of their growth properties and initial morphological characteristics, as well as to the absence of the cytopathic effect of the virus. This indicated a high sensitivity of the virus of porcine gastroenteritis virus to the action of the studied drugs. The results obtained make it possible to consider the compounds being studied as promising antiviral agents.
Keywords: transgenic porcine gastroenteritis virus, cytopathic effect, antiviral activity, antimicrobial properties, synthetic compounds, heterocyclic compounds of the phenylpentendione series, biocompatible polymeric compound, subtoxic concentrations.
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Tikhomirova Elena I., D.Sc. in Biology, Professor, Head of the Department of Ecology of the Yuri Gagarin Stavropol State Technical University; 77, Politechnicheskaya st., Saratov, 410054; phone: 8-452-998530; e-mail: email@example.com.
Laskavyy Vladislav N., D.Sc. in Veterinary Medicine, Honored Veterinarian of the Russian Federation, Director for Science of Scientific Research Institute of Medical and Veterinary Biotechnologies; 5, 9, Akademika Koroleva st., Moscow, 129515; phone: 8-915-1100167; e-mail: firstname.lastname@example.org.
Kuznetsova Anna E., Ph.D. in Biology, Senior Researcher of the Scientific Research Institute of Medical and Veterinary Biotechnologies; 5, 9, Akademika Koroleva st., Moscow, 129515; phone: 8-961-6420874; e-mail: shirokova-7575@ mail.ru.
Vakaraeva Malika M., Ph.D. in Biology, senior lecturer of the Department of Normal and Pathological Physiology of the Medical Institute of the Chechen State University; 32, A. Sheripova st., Grozny, Chechen Republic, 364907; phone: 8-9389931011; e-mail: email@example.com.
Responsible for correspondence with the editorial board: Nechaeva Olga V., Ph. D. in Biology, Docent of the Department of Ecology of the Yuri Gagarin Saratov State Technical University; 77, Politechnicheskaya st., Saratov, 410054; phone: 8-927-1081108; e-mail: firstname.lastname@example.org.