Study of some biochemical parameters of blood of mediterranean tortoise nikolsky (testudo graeca nikolskii, 1986) in semi-free conditions of safari park

UDC 619:616.995.1-08]:598.132.4

Peretokina E.I., Lysenko A.A., Beretar I.M.

Summary. Biochemical indices of blood of Red Book species tortoise Nikolsky (Testudo Graeca Nikolskii, 1986) gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), urea, and chlorides were studied by the authors in semi-free conditions for the first time. Four turtles of 18 (22.2%) showed almost twice above AST normal in blood serum. Results of biochemical studies suggests violation of the functional state of the liver reptiles. Chloride index is within the physiological standards – 98-107 mg / dL in the majority of the surveyed turtles. However, this index in 2 turtles (11.1%) is higher than normal by 18.8 mmol/l and 32,2 mmol/l, respectively, which reveals the signs of renal insufficiency. Index of lactate dehydrogenase (LDH) above the standard between 24% to 56% In 5 of 18 of turtles (27.8%). It gives the basis to state these reptiles of liver disease and cardiovascular system. Index of GGT gamma-glutamyl transpeptidase, is within the physiological norms (0-45ME/l) in all surveyed turtles. However, index exceeded 20 or more times in the study group performance in 4 turtles (22.2%). It gives the basis to conduct additional studies of the functional status of these turtles to prevent their bones or hepatic disease. Such index as urea in the blood of all examined turtles (n=18) ranged from 0.5 to 1.6 mmol/l. This variation of indice can be taken as the norm in turtles and used in the characterization of renal function.

Keywords: turtle Nikolsky, Red Book species, safari park, biochemical indices, gamma-glutamyl transferase, lactate dehydrogenase, aspartate aminotransferase, urea, chloride, clinical examination.

References:

1. Ananyeva N.B., Orlov N.L. Atlas presmykaiushchikhsia Evrazii [Atlas of reptiles of Eurasia]. – Spb. – 2004. – s.232.

2. Ananyeva N.B., Milto K.D., Ostrovskikh S.V., Pestov G.M., Pestov M.V. Proekt po izucheniiu i okhrane sredizemnomorskoi cherepakhi (Testudo graeca Nikolskii) na Zapadnom Kavkaze – pervye itogi i perspektivy [Project for the study and protection of Mediterranean tortoise (Testudo graeca Nikolskii) in the Western Caucasus: first results and prospects]. – 2008: pp. 25-30.

3. Bondarenko D.A. Vzaimootnosheniia Sredneaziatskoi cherepakhi (Agrionemys horsfieldii ) s parazitami v prirode [Relations between Central Asian tortoise (Agrionemys horsfieldii) and parasites in nature]. – Zoologicheskii zhurnal. – Moscow, 2015 (94 (7)). – pp. 801-815.

4. Galliamov R.R., Leontieva O.A. Biotopicheskoe raspredelenie sredizemnomorskoi cherepakhi (Testudo graeca Nikolskii) na severo-vostochnom poberezhe Chernogo moria [Habitat distribution of Mediterranean tortoise (Testudo graeca Nikolskii) in the north-eastern coast of the Black Sea]. – Moscow, 2000. – pp. 92-99.

5. Leontieva O.A., Pestov M.V., Pereshkolnik S.L. Sovremennoe sostoianie i problemy okhrany Testudo graeca Nikolskii na severo-zapade areala [Current state and problems of protection Testudo graeca Nikolskii in the northwest area]. – Voprosy gerpetologii. – Minsk, 2012. – pp. 155-159.

6. Leontieva O.A., Sidorchuk E.A. Sostav populiatsii i morfologicheskie kharakteristiki sredizemnomorskoi cherepakhi na poluostrove Abrau [Composition of population and morphological characteristics of the Mediterranean turtle on the Abrau Peninsula]. – MGU. – Moscow, 2002. – pp. 90-98.

7. Lysenko A.A., Peretokina E.I., Ponomarenko Iu.Iu. Cherepakha Nikol’skogo (Testudo graeca Nikolskii, 1986): gelmintozy, ikh profilaktika i lechenie v usloviiakh poluvol’nogo soderzhaniia [Turtle Nikolsky (Testudo graeca Nikolskii, 1986) helminthoses and their prevention and treatment in conditions of semidetention]. – Krasnodar, 2015. – pp. 172-175.

8. Pestov M.V., Malandziia V.I., Mil’to K.D. Sredizemnomorskaia cherepakha Nikol’skogo (Testudo graeca Nikolskii) v Abkhazii [Mediterranean tortoise Nikolsky (Testudo graeca Nikolskii) in Abkhazia]. – Sovremennaya gerpetologiia. – Saint-Petersburg, 2009 (9). – pp. 41-51.

9. Peretokina E.I. Veterinarnye aspekty sokhraneniia krasnoknizhnogo vida cherepakh Testudo graeca (Nikolskii, 1986) [Veterinary aspects of conservation of Red Book species of turtles Testudo graeca (Nikolskii, 1986)]. – Krasnodar, 2015. - pp. 167-175.

10. Peretokina E.I., Lysenko A.A. Lechenie i profilaktika gel’mintozov sredizemnomorskoi cherepakhi Nikol’skogo Testudo graeca (Nikolskii, 1986) [Treatment and prevention of helminthoses of Mediterranean tortoise]. – Krasnodar, 2015. – pp. 175-182.

11. Shchukina I.V., Stepovoi A.V., Borisenko V.V., Nikolaenko V.I. Perspektivy ispol’zovaniia mikrobiologicheskikh preparatov [Perspectives of use of microbiological preparations]. – 2015 (85): pp. 25-28.

12. Volkova S.A., Stepovoi A.V., Borisenko V.V., Nikolaenko V.I. Probioticheskaia kormovaia dobavka s antibioticheskimi svoistvami dlia ptitsevodstva [Probiotic feed additive with antibiotic properties for poultry]. – 2015 (85): pp. 4-6.

13. Tuniev B.S. Presmykaiushchiesia Reptilia [Reptiles]. – Maykop, 2000. – pp. 313-320.

Author affiliation:

Peretokina Elena I., post-graduate student of the department of pharmacology and therapy of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-221-58-20; e-mail: safari-park-gel@yandex.ru.

Beretar Inna M., Ph.D. in Veterinary Medicine, head of the antiparasitic and veterinary-sanitary measures of the Krasnodar regional station of fighting against animal diseases; 15/1, Kalinina st., Krasnodar, 350004; phone: 8-861-221-62-39; e-mail: bertarinna@gmail.com.

Responsible for correspondence with the editorial board: Lysenko Aleksandr A., D.Sc. in Veterinary Medicine, professor of the department of pharmacology and therapy of the Kuban State Agrarian University; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-221-58-20; e-mail: vet.kubgau@mail.ru.