Diagnostics of animal rabies by enzyme immunoassay. Comparison of direct and indirect sandwich Elisa options

Sukharkov A.Yu., Nazarov N.A., Metlin A.E.

Summary

Diagnostics of rabies is important to provide timely post-exposure medical care for people and rabies control among animals. Currently, basic diagnostic methods of rabies are: reaction of immunofluorescence and biological test in mice, as well as virus isolation in cell culture. The main disadvantage of biological tests in mice and virus isolation in cell culture is duration of the study. The authors have developed a direct sandwich ELISA option for diagnostics of rabies. Developed solid-phase direct sandwich enzyme immunoassay option for the diagnostics of animal rabies, based on polyclonal antibodies to rabies virus ribonucleoprotein, and comparative trials of the direct method with the indirect sandwich ELISA variant, previously developed. Probation of both methods showed similar results in 100% of cases. Diagnostic sensitivity and specificity was respectively 97,14% and 100%, respectively. In case of equal diagnostic value of direct sandwich option has an advantage over indirect, as more and express less costly method.

Key words: rabies, rabies virus ribonucleoprotein, diagnostics of rabies, ELISA, brain, rabies peroxidate conjugate.

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Author affiliation

Nazarov Nikolay A., Ph.D. in Biology, leading scientific researcher of the All-Russian Research Institute of Animal Health; e-mail: nazarov_na@arriah.ru.

Metlin Artem E., Ph.D. in Veterinary Medicine, laboratory manager of the All-Russian Research Institute of Animal Health (Federal Centre for Animal Health); e-mail: metlin@arriah.ru.

Responsible for correspondence with the editorial board: Sukharkov Andrey Yu., postgraduate student, leading biologist of the All-Russian Research Institute of Animal Health; mcrd. Yurevets, Vladimir, 600901; phone: (4922) 26-38-77, (4922) 26-06-14, (4922) 26-19-14; e-mail: andrey_33rus@mail.ru.