ÓÄÊ 578.823:619
DOI 10.33861/2071-8020-2024-5-17-19
Veretennikov V. V., Dzhavadov E. D., Tarlavin N. V., Kraskov D. A., Rumyantsev A. M., Ishtuganova V. V.
Summary. Currently, there are many known methods used to determine the antibody titer to infectious bursal disease virus, but the enzyme immunoassay, using the whole virion of the virus as antigen, is the most popular. In this study, we investigated the use of cell lysate and VP2 protein of IBD virus expressed in bacterial cells as a possible alternative to these kits in terms of safety and cost-effectiveness. The proteins in the cell lysate as well as in purified form were used (at concentrations of 1 pg and 5 pg per well) to perform indirect ELISA using positive and negative sera. ELISA results using purified VP2 correlated with those of the commercial kit. Cell lysate did not perform as well as purified VP2. Thus, ELISA based on purified recombinant VP2 protein is a good alternative to traditional ELISAs that use whole virus virions as antigen.
Keywords: poultry farming, infectious bursal disease, recombinant VP2 protein, enzyme immunoassay.
Author affliation:
Dzhavadov Eduard D., D. Sc. in Veterinary Medicine, Professor of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-921-9666774; e-mail: vnivip1@mail.ru.
Tarlavin Nikolay V., Ph. D. in Veterinary Medicine, Assistant of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8430353; e-mail: tarlav1995@bk.ru.
Kraskov Dmitry A., postgraduate student of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-921-4152266; e-mail: kraskov-00@bk.ru.
Rumyantsev Andrey M., Ph. D. in Biology, Scientific Researcher of the Department of Genetics and Biotechnology of the Saint-Petersburg State University; 7-9, Universitetskaya emb., Saint-Petersburg, 199034; phone: 8-911-2654652; e-mail: rumyantsev-am@mail.ru.
Ishtuganova Valeriya V., student of the Department of Genetics and Biotechnology of the Saint-Petersburg State University; 7-9, Universitetskaya emb., Saint-Petersburg, 199034; phone: 8-981-7473464; e-mail: st086860@student.spbu.ru.
Responsible for correspondence with the editorial board: Veretennikov Vladislav V., Ph. D. in Veterinary Medicine, Assistant of the Department of Epizootology named after V. P. Urban of the Saint-Petersburg State University of Veterinary Medicine; 5, Chernigovskaya st., Saint-Petersburg, 196084; phone: 8-981-8778769; e-mail: vlad.veretennikov.96@mail.ru.
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