ÓÄÊ 579. 843. 2
DOI 10.33861/2071-8020-2023-5-43-45
Yakovenko P. P., Tishchenko A. S., Maksim E. A., Koshchaev A. G., Alferov D. O., Kaloshkina I. M.
Summary. Results of the microbiological study of isolated from aquaculture objects Aeromonas genus bacteria in order to establish their sensitivity to various classes of antibacterial drugs using the disk diffusion method are presented in the article. The authors used selective and differential nutrient media in order to obtain the pure culture of bacteria of the Aeromonas genus from pathological fish material. During the study, the antagonistic activity of probiotic microorganisms against bacteria of the Aeromonas genus isolated from the studied fish was also established. As a result of the studies, it was possible to establish the presence of sensitivity in the studied species of bacteria of the Aeromonas genus to the antibacterial drugs ciprofloxacim, impinem, cefaclor. The sensitivity of aeromonads to these drugs was established in 100% of cases. In addition, the studied bacterium of the species Aeromonas Ichthiosmia LT4159 showed sensitivity, in addition to the above-mentioned antibacterial drugs, to the drug amoxicillin. When conducting antagonistic activity of probiotic microorganisms isolated directly from the intestines of fish, a high degree of sensitivity was established in the bacterium Lapidilactobacillus dextrinicus.
Keywords: aquaculture, bacteria of the genus Aeromonas, aeromonas, strain, antibacterial drugs, antagonistic activity, probiotic microorganisms, sensitivity, antibiotic resistance.
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Author affiliation:
Tishchenko Aleksandr S., Ph. D. in Veterinary Medicine, Head of the Microbiology Laboratory of the Biotechnology Center, docent of the department of microbiology, epizootology and virology of the Kuban State Agrarian University named after I. T. Trubilin; 13, Kalinina st., Krasnodar, 350044; phone: 8-928-4692399; e-mail: mephisto83@inbox.ru.
Maksim Ekaterina A., Ph. D. in Biology, Head of the Innovation and Technology Center for Aquaculture of the Kuban State Agrarian University named after I. T. Trubilin; 13, Kalinina st., Krasnodar, 350044.
Koshchaev Andrey G., D. Sc. in Biology, professor, Academician of the Russian Academy of Sciences, professor of the department of biotechnology, biochemistry and biophysics of the Kuban State Agrarian University named after I. T. Trubilin; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-2215637; e-mail: kagbio@mail.ru.
Alferov Dmitry O., student of the faculty of Veterinary Medicine of the Kuban State Agrarian University named after I. T. Trubilin; 13, Kalinina st., Krasnodar, 350044.
Kaloshkina Inna M., Ph.D. in Veterinary Medicine, head of the department of antiparasitic, veterinary and sanitary measures of the Krasnodar regional station of fighting against animal diseases; 15/1, Kalinina st., Krasnodar, 350004; phone: 8-861-2216091; e-mail: beretarinna@gmail.com.
Responsible for correspondence with the editorial board: Yakovenko Pavel P., Ph.D. in Veterinary Medicine, docent of the department of microbiology, epizootology and virology of the Kuban State Agrarian University named after I.T. Trubilin; 13, Kalinina st., Krasnodar, 350044; phone: 8-900-2360273; e-mail: pavel2912@mail.ru.
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