UDC 363.7-092.9:616.72-007.17-008.8:612.398.12:612.015.31
Spirkina E.S., Matveeva E.L., Gasanova A.G., Stepanov M.A.
Summary. Experimental osteoarthritis of the stifle joint in dogs was modeled by immobilization of both stifle joints with the Ilizarov apparatus and dissection of the femoral artery. The studied material was blood serum of 30 dogs. Ten dogs were intact, 14 with an early stage of osteoarthritis (28 days of the experiment), and six - with a late stage of osteoarthritis (56 days of the experiment). Osteoarthritis was defined according to clinical and radiographic signs in the joints. Biochemical parameters were studied at the stages of the experiment. Purpose of the study was to reveal the changes in lipid peroxidation (LPO) and protein oxidation modification (POM) in the experimental animals at different stages of the experiment. The results of this study showed statistically significant changes in the serum content of the dogs at different stages of the pathological processes. A decrease in the concentrations of primary and secondary products of lipid peroxidation in the late stage was noted while it was not observed in the early OA stage. The concentrations of protein aldehydes were reduced in the products of oxidative modification both at early and late stages of the pathological process. A statistically significant increase in the concentrations of a2-globulin, p-globulin and y-globulin fractions indicates the presence of inflammation and chronic forms of the disease. The results obtained in the study may be used for early diagnosis of the pathological process in major joints and be an additional information when diagnosis is established.
Keywords: dogs, osteoarthritis, lipid peroxidation, oxidative modification of proteins, degenerative-dystrophic changes in joints, blood serum, diene conjugate, malondialdehyde, total protein, total lipids, aldehydes, ketones
References:
1. Borisov M.S. Diagnostika, lechenie, profilaktika zakrytykh i otkrytykh povrezhdniy sustavov i sukhozhiliy u zhivotnykh [Diagnosis, therapy, prevention of closed and open injuries of joints and tendons in animals]. - Moscow, 2001. - 43 p.
2. Vyushin A.V., Waido A.I., Gerasimova I.A. Protsessy perekisnogo okisleniya belkov u krys, selektivnykh po porogu vozbudimosti nervnoy sistemy [Peroxidation of proteins in rats by selective threshold of excitability of nervous system]. - 2002 (133 (3)). - pp. 292-296.
3. Davydov V.B. Metody eksperimentalnogo modelirovaniya osteoartrozov u melkikh eksperimentalnykh zhivotnykh [Methods of experimental osteoarthritis modeling in small test animals]. - www.allvet.ru/modules/articles/article. php?id=31 (reference date of 25.07.2010).
4. Dedukh N.V., Malyshkina S.V., Pankov Ye.Ya. Metod morfologicheskoy otsenki sustava pri destruktivno-distroficheskikh porazheniyakh [Method of morphological joint assessment at destructive-dystrophic lesions]. - Kyiv, 1988. - p. 2.
5. Korolyuk M.A., Ivanova L.I., Tokarev V.Ye. Metod opredeleniya aktivnosti katalazy [Method for determining catalase activity]. - Lab. delo. - Moscow, 1988 (1). - pp. 16-19.
6. Makushin V.D., Stepanov M.A., Stupina T.A. Modelirovanie osteoartroza kolennogo sustava u sobak [Modeling of stifle joint osteoarthritis in dogs]. -Biomedicine. - Svetlye gory, 2012 (3). - pp. 108-115.
7. Orekhovich V.N. Sovremennye metody v biokhimii [Modern methods in biochemistry]. - Meditsina, 1977. - p. 392.
8. Makushin V.D., Stepanov M.A., Stupina T.A. Sposob modelirovaniya osteoartroza kolennogo sustava [Modeling method of stifle joint osteoarthritis]. - Pat. 2452999. - 2011 (16).
9. Shevtsov V.I., Asonova S.N., Gracheva L.I. Modelirovanie sustavnoy patologii putem immobilizatsii sustavov apparatom Ilizarova [Modeling of articular pathology by immobilizing of joint by Ilizarov apparatus]. - Kazan, 1994. - p. 35.
10. Yagnikov S.A., Mitin V.N. Konservativnoe lechenie osteoartroza krupnykh sustavov u sobak [Conservative therapy of osteoarthritis of large joints in dogs]. - 2004: 7-11.
11. Vide supra.
Author affiliation:
Matveeva Elena L., D.Sc. in Biology, leading scientific researcher of the laboratory of biochemistry of the Ilizarov Russian Scientific Center; 6, Ulyanova st., Kurgan, 640014; phone: 8(3522)45-05-38; å-mail: matveevan@mail.ru.
Gasanova Anna G., junior scientific researcher of the laboratory of biochemistry of the Ilizarov Russian Scientific Center; 6, Ulyanova st., Kurgan, 640014; phone: 8(3522)45-05-38; å-mail: gasanova08@mail.ru.
Stepanov Mikhail A., Ph.D. in Veterinary Medicine, leading scientific researcher, veterinarian of the laboratory of purulent osteology and replacement of defects of limbs of the Ilizarov Russian Scientific Center; 6, Ulyanova st., Kurgan, 640014; phone: 8(3522) 45-46-36; å-mail: m-stepanov@mail.ru.
Responsible for correspondence with the editorial board: Spirkina Elena S., assistant researcher of the laboratory of biochemistry of the Ilizarov Russian Scientific Center; 6, Ulyanova st., Kurgan, 640014; phone: 8 (3522) 45-05-38; å-mail: spirkina.82@mail.ru.
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