Correction methods of superovulation induction to obtain optimal number of embryos suitable for embryo transfer

UDC 619:618:615.37:636.2

Kosovskii G.Iu., Popov D.V., Brigida A.V.

Summary. Use of superovulation induction technology and embryo transfer is an ubiquitous practice in cattle breeding. Three different types of gonadotropins are used for initiation of superovulation in cows: gonadotropin-releasing hormone extracted from porcine hypophysis and hypophysises of other farm animals (FSH); equine chorionic gonadotropin (eCG); human menopausal gonadotropin (hMG). Currently advantages of FSH treatment compared to eCG are widely recognized in the embryo transfer industry. However all current techniques follicular wave and ovulation regulation still cannot fully eliminate variability of the response to superovulation induction. In present work the research results of thymalinum immunomodulator application as preparation step for donor cows before the induction of superovulation with FSH are shown. Experimental animal groups were formed on the basis of pairs of peers based on the physiological state, age, efficiency, live weight and the stage of sexual cycle. Authors noted that an increase in the duration of estrus increases the number of washed embryos, suggesting an increase in the number of ovulations. Obtained results allow us to analyze and recommend introduction order of superovulation inducing agents that will increase positive reaction to the inductors and will lead to generation of an optimal number of embryos suitable for embryos transfer.

Key words: large horned cattle, cow donor, transplantation, embryos, superovulation, synchronization of oestrus, sexual cyclicity, gonadotropin, follicle oocyte fertilization.

References:

1. Babenkov V.Iu. Sovershenstvovanie sposoba indutsirovaniia superovuliatsii u korov-donorov [Inducing superovulation improving method in cows donors]. – Gorki, 1991. – pp. 6-8.

2. Madison V., Madison L. V pomoshch novomu skotovodu [To help new cattlemen]. – Molochnoe i myasnoe skotovodstvo. – Balashikha, 2003 (4). – pp. 10-16.

3. Malinin V.V., Morozov V.G. Mekhanizmy peptidnoi reguliatsii gomeostaza [Mechanisms of peptide regulation of homeostasis]. – Moscow, 2004. – pp. 7-18.

4. Popov D.V., Bezborodov N.V. Povyshenie kachestva embrioproduktivnosti u korov donorov [Improving the quality of the embryo production in cows donors]. – Orel, 2011.

5. Popov D.V. Bezborodov N.V. Sposob vyzyvaniia superovuliatsii u korov donorov [Superovulation inducing method in cows donors]. – Vestnik Altayskogo gosudarstvennogo agrarnogo universiteta. – Barnaul, 2010 (9 (71)).

6. Ptashinskaia M. Kratkoe rukovodstvo po reproduktsii zhivotnykh [Animal Reproduction Guide]. – 2009.

7. Ternovykh S.V. Fiziologicheskie osnovy povysheniia effektivnosti gormonalnoi induktsii poliovuliatsii u korov-donorov embrionov [Physiological basis for improving the efficiency of hormonal induction of polyovulation in cows donor embryos]. – Voronezh, 2001.

8. Betteridge KJ. Farm animal embryo technologies: Achievements and perspectives. Theriogenology 2006; 65: 905-913.

9. Chebel RC., Demetrio DGB., Metzger J. Factors affecting success of embryo collection and transfer in large dairy herds. Theriogenology 2008;69:98-106.

10. Ginther OJ., Beg MA., Donadeu FX., Bergfelt DR. Mechanism of follicle deviation in monovular farm species. Anim Reprod Sci 2003; 78:23 9–25 7.

11. Scherzer J., Fayrer-Hosken RA., Ray L., Hurley DJ., Heusner GL. Advancement in large animal embryo transfer and related biotechnologies. Reprod Domest Anim 2008;43: 371-6.

Author affiliation:

Popov Dmitrii V., scientific researcher of the Federal State Budget Scientific Institution "Center of experimental embryology and reproductive biotechnologies"; 4/12, Kostiakova st., Moscow, 125480; e-mail: info-ceerb@mail.ru.

Brigida Artem V., scientific researcher of the Federal State Budget Scientific Institution "Center of experimental embryology and reproductive biotechnologies"; 4/12, Kostiakova st., Moscow, 125480; e-mail: info-ceerb@mail.ru.

Responsible for correspondence with editorial: Kosovskii Gleb Iu., D.Sc. in Biology, head of the Federal State Budget Scientific Institution "Center of experimental embryology and reproductive biotechnologies"; 4/12, Kostiakova st., Moscow, 125480; phone: 8-495-610-21-31; e-mail: info-ceerb@mail.ru.


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