UDC 619: 616: 98: 578.
Gendzhieva O.B., Rudenko A.V., Basangova R.V., Ubushieva A.V., Gendzhiev A.Ia., oiseikina L.G.
Summary. Regular full charges of ticks from animals during the year defined seasonal population dynamics of ticks in a certain area. Molecular genetic analysis of ticks for the detection of proviral DNA of bovine leucose virus was conducted. Computerized diagnosis system for detection of proviral DNA of bovine leucose in ticks and animal blood using instrument Rotor-Gene 6000 Real-time was applied. Polymerase chain reaction with hybridization fluorescence detection was used to identify the DNA provirus. Proviral DNA of bovine leucose virus (BLV) in Ixodes ticks at the territory of the Republic of Kalmykia was studied in PCR in realtime. Also the analysis of the blood of test animals by PCR in real time was made for the purpose of detecting carriers of the pathogen of BLV. Specific part of the DNA of bovine leucose provirus was detected in Ixodidae ticks Hyalomma marginatum and Hyalomma scupense. Serological and genetic testing confirmed the infection of cows with bovine leukemia. It revealed that BLV proviral DNA was found in ticks of the species Hyalomma marginatum, Hyalomma scupense in farms where cows are reacting to BLV in PCR analysis. Frequency of provirus in ticks and the number of cows that respond in PCR for BLV was determined. PCR analysis of blood of cows for the presence of BLV showed that there is a relationship between the frequency of occurrence of the BLV provirus and the number of cows, positively responding to the virus.
Key words: ticks, Íyalomma marginatum, Íyalomma scupense, molecular analisis, polymerase chain reaction, detection, proviral DNA, vector-borne path, bovine leucose virus, infection, epizootology.
References:
1. Amirokov M.A. Kompleksnaia otsenka faktorov, vliiaiushchikh na osobennosti proiavleniia i rasprostraneniia leikoza krupnogo rogatogo skota, i sovershenstvovanie sistemy, obespechivaiushchei epizooticheskoe blagopoluchie [Integrated assessment of factors influencing the features of display and distribution of bovine leukosis, and improving of the epizootic welfare ensuring]. – Barnaul, 2011. – 39 p.
2. Apalkin V.A. Ekologo-epizootologicheskoe obosnovanie razrabotki i realizatsii novoi skhemy veterinarno-profilakticheskikh meropriyatii v Altaiskom krae [Ecological and epidemiological substantiation of development and implementation of the new scheme of veterinary preventive measures in Altai region].– Novosibirsk, 1998. – 45 p.
3. Busol V.A., Andriian E.A., Tsymbal V.I., Khokhlov V.A., Enenko N.G. Leykozogennost krovi, moloka i vydelenii bolnykh leikozom i seropozitivnykh zhivotnykh [Leucosis geneity of blood, milk and secretions of animals with leukemia and seropositive animals]. – DonGau. – Persianovskii, 1990. – pp. 12-16.
4. Valikhov A.F., Shishkov V.P., Burba L.G. Leikoz krupnogo rogatogo skota (virusologicheskie aspekty) [Bovine leukosis (virological aspects)]. – Moscow, 1977. – 23 p.
5. Ganiev I.M. Ekologo-faunisticheskie issledovaniia iksodovykh kleshchei i epizootologiia gemosporidiozov selskokhoziaistvennykh zhivotnykh Zapadnogo Prikaspiia [Ecological and faunal studies ixodid ticks and epizootology of haemosporidiosis of agricultural animals of the Western Caspian].– Nauka. – Moscow, 1966. – pp. 61-62.
6. Gendzhieva O.B., Guliukin M.I. Epizootologiia leikoza v miasnom skotovodstve [Epizootology of leucose in beef cattle breeding]. – Veterinariia. – Moscow, 2012 (7). – pp. 23-26.
7. Gendzhieva O.B. Effektivnost ozdorovitelnykh meropriyatii pri leikoze krupnogo rogatogo skota na territorii Respubliki Kalmykiia [Efficiency of health measures at bovine leucoseon the territory of the Republic of Kalmykia]. – Veterinariia i kormlenie. – Moscow, 2010 (3). – pp. 22-23.
8. Guliukin M.I., Valikhov A.F., Nakhmanson V.M., Ivanova L.A., Grek K.P., Lopunov S.V. Osobennosti infektsionnogo protsessa, indutsirovannogo virusom leykoza krupnogo rogatogo skota [Features of bovine leukemia virus-induced infectious process]. – VIEV. – Moscow, 2008. – pp. 106-113.
9. Dzhupina S.I. Epizooticheskii protsess leikoza krupnogo rogatogo skota I perspektivy devastatsii vozbuditelia infektsii [Epizootic process of leucose of large horned cattle and prospects of pathogen devastation].– Veterinarnaia patologiia. – Novocherkassk, 2014 (1). – pp. 98-103.
10. Dmitriev A.F., Beliaev V.A., Tregubov A.N. Rekomendatsii po nedopushcheniiu rasprostraneniia virusa leikoza neblagopoluchnymik hoziaistvami na osnove primeneniia sovershenstvovannykh protivoleikoznykh meropriiatii
[Recommendations for prevention of the spread of the virus of leukemia by disadvantaged farms on the basis of the application of anti leukemic measures]. – Stavropol, 2014. – 48 p.
11. Karan L.S., Koliasnikova N.M., Toporkova M.G., Makhneva M.A., Nadezhdina M.V. Primenenie PTSR v rezhime realnogo vremeni dlia diagnostiki razlichnykh kleshchevykh infektsii [Use of PCR in real-time for diagnosis of various mite infection].– Mikrobiologiia. – Moscow, 2010 (3). S. 72-77
12. Loginov S.I., Khramtsov V.V., Vysochin A.V., Tabakaev V.V. Epizootologicheskie pokazateli dlia otsenki proiavleniia epizooticheskogo protsessa leikoza krupnogo rogatogo skota [Epizootological indicators to assess symptoms of epizootic bovine leukosis]. –Vestnik NGAU. – Novosibirsk, 2011 (1 (17)).– pp. 96-100.
13. Mager S.N., Smirnov P.N., Khramtsov V.V., Osipova N.A., Agarkova T.A. Mery profilaktiki i borby s leikozom krupnogo rogatogo skota v lichnykh podsobnykhk hoziaistvakh grazhdan [Measures for the prevention and control of bovine leukemia in private farms of citizens]. – Vestnik NGAU. – Novosibirsk, 2011. № 1 (17). – pp. 100-104.
14. Rudenko A.V. Profilaktika protozoinykh zabolevanii v promyshlennykh kompleksakh [Prevention of protozoal diseases in industrial complexes]. – Elista, 1985. – pp. 86-92
15. Rudenko A.V., Goriaev N.E. Iksodovye kleshchi – parazity zhivotnykh Kalmykii [Ixodid ticks – parasites of animals in Kalmykia]. – Elista, 2006. – pp. 79-82.
16. Sivkov G.S., Glazunov Iu.V., Podshivalov D.A., Stepanov A.G., Donnik I.M., Tatarchuk A.T Izuchenie roli iksodovykh kleshchey v peredache virusa leykoza krupnogo rogatogo skota [Studying the role of ixodid ticks in transmission of bovine leukemia].– Veterinariia. – Moscow, 2009 (12). – pp. 14-17.
17. Eaves F.W. Afield evaluation of the polymerase chain reaction procedure for the detection of bovine leukemia virus pro viral DNA in cattle./ F.W.Eaves, Â.Molloy,C.K. Dimmock,L.E. Eaves //Vet. Microbiol.1994.39. P. 313 – 321.
18. Gillet N. Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. / N. Gillet, A. Florins, Boxus M. ,C. Burteau, A. Nigro, F. Vandermeers, H. Balon,A.-B. Bouzar, J. Defoiche, A. Burny, M. Reichert, R. Kettmann, L.Willems // J. Retrovirol.2007.N4.P.18-50.
19. Kurdi A. Serologic and virologic investigations on the presence of BLVinfections in a dairy herd in Syria./ A. Kurdi, P. Blankenstein. // Berl Munch. TierarztlWochenschr, 1999. Jan.,l12(1).P. 18-23.
20. Lewin H. Evidence for BoLA-linked resistance and susceptibility to subclinical progression of bovine leukaemia virus infection/ H. Lewin, D. Bernoco //Animal Genetics. 1986. V. 17. P. 197-207.
21. Mammericx M. Rapid Detection of Bovine Leukemia Virus Infection in a large Cattle population with an ELISA performed on Pooled Sera GroupedBy Herd. J./ M. Mammericx, D. Portetelle,I. Nys, A.Bumi //Vet.Med.-1985.-V.32.P.601-608.
Author affiliation:
Rudenko Anatolii V., docent of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista,358000; phone: 8(84722)41005; e-mail: uni@kalmsu.ru.
Basangova Rada V., post-graduate student of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista, 358000; phone: 8(962)773-98-28; e-mail: basangova.r@yandex.ru.
Ubushieva Altana V., post-graduate student of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista, 358000; phone: 8(927)598-05-67; e-mail: ameli-altanas@mail.ru.
Gendzhiev Aleksander Ia., post-graduate student of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista, 358000; phone: 8(84722)41005; e-mail: uni@kalmsu.ru.
Moiseikina Liudmila G., professor of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista, 358000; phone: 8(84722)41005; e-mail: uni@kalmsu.ru.
Responsible for correspondence with the editorial board: Gendzhieva Olga B., docent of the department of zootechnics and veterinary medicine of the Kalmyk State University; 11, Pushkina st., Elista, 358000; phone: 8(927)645-87-74; e-mail:gend_olga@mail.ru.
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