Genotyping of BLV isolates spread at the territory of the Republ

Gulyukin M.I., Kozyreva N.G., Gendzhieva O.B., Ivanova L.A.

Summary

Amplification of the desired fragment of the pol gene was carried out with the use of developed primers PF2-PR2 complementary to a section of the BLV pol gene. The 438 bp fragments of the genome of BLV provirus isolates from mature seropositive cows were obtained by the PCR method. The nucleotide sequence of a section of the provirus pol gene of bovine leukemia virus (BLV) isolates from animals from some farms in Gorodovikovsky region of the Republic of Kalmykiya is determined whereby permit concluding homology of circulating viruses and belong to the same group as BLV isolates from Argentina, Australia, Brazil, and the United States. On the basis of the revealed homology of the section of the pol gene of BLV isolates, primers PF2-PR2 can be used also for detecting BLV proviral DNA when conducting routine PCR diagnosis of bovine leucosis.

Key words: bovine leucosis virus, PCR, sequencing, phylogenetic analysis.

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Author affiliation

Gulyukin Mikhail I., D.Sc. in Veterinary Medicine, professor, academician of the Russian Academy of Agricultural Sciences, director of the Kovalenko All-Russian Institute of Experimental Veterinary Medicine; 24/1, Ryazansky ave., Moscow, 109428; phone: 8(495)9700368; e-mail: viev@mail.ru.

Ivanova Lyudmila A., Ph.D. in Biology, leading scientific researcher of the laboratory of leucosis of the Kovalenko All-Russian Institute of Experimental Veterinary Medicine; 24/1, Ryazansky ave., Moscow, 109428; phone: 8(495)9700366; e-mail: viev@mail.ru.

Gendzhieva Olga B., Ph.D. in Veterinary Medicine, senior lecturer of the department of zootechny of the Kalmyk state university; phone: 8(847)2239038; e-mail: gend_olga@mail.ru.

Responsible for correspondence with the editorial board:

Kozyreva Nataliya G., junior scientific researcher of the laboratory of leucosis of the Kovalenko All-Russian Institute of Experimental Veterinary Medicine; 24/1, Ryazansky ave., Moscow, 109428; phone: 8(495)9700366, 8-915-113-75-00; e-mail: nk07-73@rambler.ru.

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