ÓÄÊ 576.8:573.6.086.83:579.842.1:579.841.1:578.825.1
DOI 10.33861/2071-8020-2026-1-32-36
Original Empirical Research
Romanova N. N., Tokareva S. B., Shchelkunova Yu. P.
Abstract. Disease outbreaks caused by circulating pathogens in aquaculture lead to high mortality rates, creating a pressing need for fish pathologists to develop rapid diagnostic methods and targeted interventions, such as vaccines and bacteriophages. The emerging collection of fish disease pathogens in the ichthyopathology laboratory of the Freshwater Fisheries Branch of the All-Russian Research Institute of Fisheries and Oceanography includes 7 bacterial strains (Aeromonas hydrophila, A. veronii, A. salmonicida, A. eucrenophila, Pseudomonas sp., and 2 strains of Pseudomonas alcaligenes) and 6 isolates of herpesvirus (SbSHV). Bacteria strains were isolated from salmon, sturgeon, and carp fish in various types of fish farms in the Central Federal District of the Russian Federation. During storage under the following conditions: cryopreservation in liquid nitrogen (t=-196°C), freezing at ultra-low temperatures (t=-80°C), and refrigeration (t= +4 – +8°C), the bacteria showed high viability (abundant and continuous growth in 100% of the samples). The pathogenicity (DNase activity) of the aeromonad strains tended to decrease, and the decrease was most significant in the A. hydrophila bacterium. The SbSHV isolates in the collection were isolated in the regions of the Russian Federation and neighboring countries from young fish of different ages Siberian, Russian sturgeons, and their hybrids. After 3 months of storage at different incubation temperatures (+4°C; -18°C; -80°C), it was found that -80°C was the optimal temperature in a low-temperature freezer.
Keywords: bacteria, Aeromonas sp., Pseudomonas sp., Siberian sturgeon herpesvirus isolates, collection, storage.
References:
1. Vide supra.
2. Minaeva A. N., Lomakin A. A., Vasiliev D. A. et al. General characteristics of bacteria of the species Aeromonas veronii. Eurasian Union of Scientists. 2021; 2 (83): 7-9.
3. Pseudomonas (pseudomonads, bacterial genus) https://gastroscan.ru/ handbook/118/4160.
4. Holt J., Krieg N., Sneath P. et al. Bergey’s Manual of Determinative Bacteriology. Vol. 1. Mir. 1997: 432 p.
5. Vide supra.
6. Shchelkunov I. S., Shchelkunova T. I., Shchelkunov A. I. et al. Herpesvirus disease in sturgeons in Russia. Russian Veterinary Journal. Agricultural Animals. 2007; (1): 10-12.
7-9. Vide supra
10. Methodological guidelines for determining the pathogenicity of aeromonads by the degree of DNase activity. Collection of instructions for combating fish diseases. Part 1. AMB-agro Marketing Department. 1998: 150-151.
11. Methodological guidelines for the identification of viruses and laboratory diagnosis of viral fish diseases. Collection of instructions for combating fish diseases. Part 1. AMB-agro Marketing Department. 1998: 60-75.
12. Manual of Diagnostic Tests for Aquatic Animals. 8th ed. OIE. 2017.
13. Shchelkunov I. S., Shchelkunov A. I., Shchelkunova T. I. et al. Methodological recommendations for the diagnosis of herpesvirus disease in Siberian sturgeon. 2009: 10 p.
14. Baryshnikova E. I., Senina M. E., Shchelkunova Yu. P. et al. Development of a test system for detecting Siberian sturgeon herpesvirus DNA by real-time PCR. Veterinary Medicine. 2023; (2): 25-29.
15. Shchelkunova Yu. P., Baryshnikova E. I., Senina M. E. [et al.]. Sensitivity of laboratory methods for diagnosing herpesvirus disease in hybrid sturgeon. Veterinary Medicine. 2023; (3): 31-37.
16. Vide supra.
17. Sanitary rules and norms SanPiN 3.3686-21 ‘Sanitary and epidemiological requirements for the prevention of infectious diseases’ (approved by the Decree of Rospotrebnadzor on January 28, 2021, No. 4, as amended on May 25, 2022).
Author affiliation:
Tokareva Svetlana B., Chief Specialist of the Laboratory of Ichthyopathology of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 A, Rybnoe sttl., Dmitrovsky urban district, Moscow region, 141821; phone: 8-495-1086856 (ext. 132); e-mail: tokareva@vniiprh.vniro.ru.
Shchelkunova Yulia P., Ph.D. in Biology, Deputy Head of the Laboratory of Ichthyopathology of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; 40 A, Rybnoe sttl., Dmitrovsky urban district, Moscow region, 141821; phone: 8-495-1086856 (ext. 118); e-mail: schelkunova@vniiprh.vniro.ru.
Responsible for correspondence with the editorial board: Romanova Natalia N., Ph. D. in Biology, Docent, Head of the Laboratory of Ichthyopathology of the Branch for Freshwater Fisheries of the All-Russian Scientific Research Institute of Fisheries and Oceanography; Docent of the Department of Aquaculture and Ecology of the Dmitrov Fishery Industry Technological Institute (branch) of Astrakhan State Technical University; Docent of the Department of Feeding and Feed Production of the Moscow State Academy of Veterinary Medicine and Biotechnology – MVA named after K.I. Skryabin; 40 a, Rybnoe vllg., Moscow region, 141821; phone: 8-495-1086856 (+132); e-mail: romanova@vniiprh.vniro.ru.
Authors’ Contribution: the manuscript was written with the input of all authors. All authors approved the final version of the manuscript.
Conflict of Interest Statement: the authors declare no conflict of interest.
http://vetkuban.com/en/num1_202609.html