Development of ELISA-diagnostics of African swine fever

Tsibezov V.V., Terekhova Yu.O., Balandina M.V., Latyshev O.E., Grebennikova
T.V., Verkhovsky O.A., Aliper T.I., Shevkoplyas V.N.

Summary

Based on recombinant protein vp73 from African swine fever virus (ASFV) and specific monoclonal antibodies solid-phase enzyme-linked immunosorbent assay (ELISA) was developed to measure both ASFV antigen and antibody. These assays demonstrated 100% sensitivity and specificity and may be useful technique for epidemiological surveys.

Key words: African swine fever, monoclonal antibodies, enzyme-linked immunosorbent assay.

References

  1. Costard S., Wieland B., de Glanville W., JoriF., Rowlands R., Vosloo W., Roger F., Pfeiffer D., Dixon L. (2009) African swine fever: how can global spread be prevented? Phil. Trans. R. Soc. B 364, 2683–2696.
  2. Freije J.M.P., Munoz M., Vinuela E. et al. (1993) High-level expression in Escherichia coli of the gene coding for the major structural protein (p72) of African swine fever virus. Gene. 123 (2), 259-262.
  3. Gallardo C., Reis A.L., Kalema-Zikusoka G., Malta J. , Soler A ., Blanco E., Parkhouse R. M. E., Leitão A. (2009) Recombinant Antigen Targets for Serodiagnosis of African Swine Fever. Clin. Vaccine Immunol. 16(7), 1012–1020.
  4. Gomez-Puertas P., Rodriguez F., Oviedo J.M. et al. (1998) The African swine fever virus protein p54 and p30 are involved in two distinct step of virus attachment and both contribute to the antibody mediated protective immune response. Virology, 243, 461-471.
  5. Reis A. L., Parkhouse R. M. E., Penedos A. R., Martins C., Leita A. (2007) Systematic analysis of longitudinal serological responses of pigs infected experimentally with African swine fever virus J.Gen. Virol. 88, 2426–2434.
  6. Rendleman, C. M. & Spinelli, F. J. (1994) The costs and benefits of African swine fever prevention. Am. J. Agric.Econ. 76, 1255–1255.
  7. Sánchez-Vizcaíno, J.M. (2006). African swine fever. In Diseases of Swine, 9th Edition, pp 93-102. Ed. A.D. Leman, B.E. Straw, W.L. Mengeling, S. Dallaire and D.J. Taylor. Iowa State University Press, Ames (Iowa, USA).
  8. Taylor D. J., editor. (2006) Diseases of Swine. 9th Ed. Iowa State University Press.
  9. Vidal M.I., Stiene M., Henkel J. et al. (1997) A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies . J. Virol. Methods. 66 (2), 211-218.
  10. World Organization of Animal Health (OIE). African swine fever. Manual of Standards for diagnostic test and vaccines, 2008.

Author affiliation

Tsibezov Valery V., Ph.D. in Biology, leading scientific researcher of the Scientific-research Institute of diagnostics and prophylaxis of human and animal diseases (SRI DPHAD); phone: 8-499-190-30-54; e-mail: tsibezov@yandex.ru.

Terekhova Yuliya O., research associate of the SRI DPHAD; phone: 8-499-190-30-54; e-mail: 23euro@mail.ru.

Balandina Marina V., Ph.D. in Biology, senior scientific researcher of the SRI DPHAD; phone: 8-499-190-30-54; e-mail: mbalandina@mail.ru.

Aliper Taras I., D.Sc. in Biology, professor, director of the NARVAC®; phone:8-499-190-30-54; e-mail: aliper@narvac.com.

Shevkoplyas Vladimir N., D.Sc. in Veterinary Medicine, professor of the department of parazitology, veterinary-sanitary examination and zoohygiene of the Kuban State Agrarian University, Honored Veterinarian of the Russian Federation; 13, Kalinina st., Krasnodar, 350044; phone: 8-861-221-58-20.

Latyshev Oleg E., Ph.D. in Biology, senior scientific researcher of the SRI DPHAD; phone: 8-499-190-75-61; e-mail: oleglat80@mail.ru.

Grebennikova T.V., D.Sc. in Biology, professor, laboratory manager of molecular biology of the SRI DPHAD; phone: 8-499-190-75-61; e-mail: t_grebennikova@mail.ru.

Responsible for correspondence with the editorial board: Verkhovsky Oleg A., D.Sc. in Biology, professor, president of the SRI DPHAD; 16, Gamalei st., Moscow, 123098; phone: 8 (499) 1907561; e-mail: info@dpri.ru.

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